In this research, three novel ultra-hydrophobic, low-viscosity, non-ionic, and heavier-than-water deep eutectic solvents (DES) based on salol and terpenes are proposed. The prepared DESs were effectively used as extraction solvents in the solidification of settled organic drop (SSOD) microextraction. Despite other hydrophobic DESs, these sustainable bio-compatible extraction mediums are compatible with GC. The ultrasonic-air agitation service for the formation of the emulsion was used to enhance the mass transfer of target analytes to extract into the fine DES droplets. Phase separation in this new enrichment method was simply achieved by centrifugation of cloudy solution at 20 °C followed by decanting the aqueous solution. The effect of the main variables on the extraction efficiency of methadone was investigated. The proposed method because of its distinctive features can be introduced as a simple and effective method for the enrichment of methadone in water and biological samples. Limits of detection derived after optimizing influential parameters ranged between 0.3 and 1.5 µg L−1. The relative standard deviations (RSD) were less than 10% and the enrichment factor values were in the range of 65–314. Recoveries obtained through methadone analysis in real samples were in the acceptable range of 91.5–105.5%.

中文翻译：

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使用基于萜烯的深共晶溶剂对沉淀的有机液滴微萃取进行超声空气辅助固化，以有效富集生物样品中的美沙酮

在这项研究中，提出了三种基于 Salol 和萜烯的新型超疏水、低粘度、非离子和重于水的低共熔溶剂 (DES)。所制备的 DES 可有效地用作沉淀有机液滴 (SSOD) 微萃取固化中的萃取溶剂。尽管存在其他疏水性 DES，但这些可持续的生物相容性提取介质与 GC 兼容。用于形成乳液的超声空气搅拌服务用于增强目标分析物的传质以提取到细小的 DES 液滴中。这种新的富集方法中的相分离是通过在 20 °C 下离心混浊溶液，然后滗析水溶液来实现的。研究了主要变量对美沙酮提取效率的影响。所提出的方法因其独特的特点可以作为一种简单有效的方法来富集水和生物样品中的美沙酮。优化影响参数后得出的检测限介于 0.3 和 1.5 µg L-1 之间。相对标准偏差 (RSD) 小于 10%，富集因子值在 65-314 范围内。通过对实际样品的美沙酮分析获得的回收率在 91.5-105.5% 的可接受范围内。相对标准偏差 (RSD) 小于 10%，富集因子值在 65-314 范围内。通过对实际样品的美沙酮分析获得的回收率在 91.5-105.5% 的可接受范围内。相对标准偏差 (RSD) 小于 10%，富集因子值在 65-314 范围内。通过对实际样品的美沙酮分析获得的回收率在 91.5-105.5% 的可接受范围内。