Cadmium (Cd) is a widespread toxic occupational and environmental pollutant, and its effect on lipid metabolism dysregulation has been observed. In this study, we utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) technologies to explore changes in the blood plasma proteins of mice exposed to Cd. From the 2-DE, 8 protein spots were screened in response to Cd exposure, and the identities of these proteins were revealed by MALDI-TOF MS. Western blotting was applied to analyze the expression of the apolipoproteins in both plasma and liver, which were consistent with Cd-induced dyslipidemia of their composed lipid. Moreover, the Cd-induced apolipoprotein ApoE upregulation was due to inhibition of autopahgic flux in the Cd exposed mice. It was further observed from the mouse liver that Cd reduced the expression of the lipid uptake receptor low-density lipoprotein receptor (LDLR), which might be responsible for the coordinated elevation in blood triglycerides and abnormal apolipoproteins. This study may provide a new insight into the mechanism of Cd-induced dyslipidemia and the risk of cardiovascular diseases.

镉 (Cd) 是一种广泛存在的有毒职业和环境污染物，已观察到其对脂质代谢失调的影响。在这项研究中，我们利用二维电泳 (2-DE) 和质谱 (MS) 技术来探索暴露于 Cd 的小鼠血浆蛋白的变化。从 2-DE 中筛选出 8 个响应 Cd 暴露的蛋白质点，并通过 MALDI-TOF MS 揭示了这些蛋白质的身份。应用蛋白质印迹分析血浆和肝脏中载脂蛋白的表达，这与 Cd 诱导的其组成脂质的血脂异常一致。此外，Cd 诱导的载脂蛋白 ApoE 上调是由于抑制了 Cd 暴露小鼠的自噬流。从小鼠肝脏中进一步观察到，Cd 降低了脂质摄取受体低密度脂蛋白受体 (LDLR) 的表达，这可能是血液甘油三酯和异常载脂蛋白协同升高的原因。该研究可能为Cd诱导的血脂异常的机制和心血管疾病的风险提供新的见解。