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The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data.
BMC Plant Biology ( IF 5.3 ) Pub Date : 2020-09-15 , DOI: 10.1186/s12870-020-02629-4
Baoyu Zhang 1, 2 , Xiujun Xie 1, 2 , Xuehua Liu 1, 2 , Linwen He 1, 2 , Yuanyuan Sun 1, 2 , Guangce Wang 1, 2
Affiliation  

Pyropia yezoensis (Rhodophyta) is widely cultivated in East Asia and plays important economic, ecological and research roles. Although inorganic carbon utilization of P. yezoensis has been investigated from a physiological aspect, the carbon concentration mechanism (CCM) of P. yezoensis remains unclear. To explore the CCM of P. yezoensis, especially during its different life stages, we tracked changes in the transcriptome, photosynthetic efficiency and in key enzyme activities under different inorganic carbon concentrations. Photosynthetic efficiency demonstrated that sporophytes were more sensitive to low carbon (LC) than gametophytes, with increased photosynthesis rate during both life stages under high carbon (HC) compared to normal carbon (NC) conditions. The amount of starch and number of plastoglobuli in cells corresponded with the growth reaction to different inorganic carbon (Ci) concentrations. We constructed 18 cDNA libraries from 18 samples (three biological replicates per Ci treatment at two life cycles stages) and sequenced these using the Illumina platform. De novo assembly generated 182,564 unigenes, including approximately 275 unigenes related to CCM. Most genes encoding internal carbonic anhydrase (CA) and bicarbonate transporters involved in the biophysical CCM pathway were induced under LC in comparison with NC, with transcript abundance of some PyCAs in gametophytes typically higher than that in sporophytes. We identified all key genes participating in the C4 pathway and showed that their RNA abundances changed with varying Ci conditions. High decarboxylating activity of PEPCKase and low PEPCase activity were observed in P. yezoensis. Activities of other key enzymes involved in the C4-like pathway were higher under HC than under the other two conditions. Pyruvate carboxylase (PYC) showed higher carboxylation activity than PEPC under these Ci conditions. Isocitrate lyase (ICL) showed high activity, but the activity of malate synthase (MS) was very low. We elucidated the CCM of P. yezoensis from transcriptome and enzyme activity levels. All results indicated at least two types of CCM in P. yezoensis, one involving CA and an anion exchanger (transporter), and a second, C4-like pathway belonging to the PEPCK subtype. PYC may play the main carboxylation role in this C4-like pathway, which functions in both the sporophyte and gametophyte life cycles.

中文翻译:

紫斑拟南芥(Rhodophyta)的碳酸盐浓度机制:转录组学和生化数据的证据。

紫斑拟南芥(Rhodophyta)在东亚广泛种植,并起着重要的经济,生态和研究作用。尽管已经从生理学的角度研究了紫纹野菜的无机碳利用,但是仍不清楚紫纹野菜的碳浓缩机理(CCM)。为了探索野牛假单胞菌的CCM,特别是在其不同生命阶段,我们追踪了在不同无机碳浓度下转录组,光合作用效率和关键酶活性的变化。光合作用效率表明,与天然配子体相比,孢子体对低碳(LC)较配子体更敏感,与正常碳(NC)条件相比,高碳(HC)的两个生命阶段的光合作用速率均增加。细胞中淀粉的数量和塑料球的数量对应于不同无机碳(Ci)浓度的生长反应。我们从18个样品中构建了18个cDNA文库(每个Ci处理在两个生命周期阶段进行了三个生物学重复),并使用Illumina平台对其进行了测序。从头大会产生了182,564个单基因,包括大约275个与CCM相关的单基因。与NC相比,大多数编码参与生物物理CCM途径的内部碳酸酐酶(CA)和碳酸氢盐转运蛋白的基因都是在LC下诱导的,配子体中某些PyCA的转录本丰度通常高于孢子体。我们鉴定了所有参与C4途径的关键基因,并表明它们的RNA丰度随着Ci条件的变化而改变。在斑节对虾中观察到PEPCKase的高脱羧活性和PEPCase的低活性。在HC下,参与C4样途径的其他关键酶的活性高于在其他两种条件下。在这些Ci条件下,丙酮酸羧化酶(PYC)显示出比PEPC更高的羧化活性。异柠檬酸裂合酶(ICL)表现出高活性,但苹果酸合酶(MS)的活性非常低。我们从转录组和酶活性水平阐明了假山疫霉的CCM。所有结果表明,至少在yezoensis中有两种CCM类型,一种涉及CA和阴离子交换剂(转运蛋白),另一种属于PEPCK亚型的C4样途径。PYC可能在这种C4样途径中起主要的羧化作用,在孢子体和配子体生命周期中都起作用。
更新日期:2020-09-15
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