Odorant-binding proteins (OBPs) play an important role in odor perception and transport in insects. However, little is known about whether OBPs perform other functions in insects, particularly in Apis cerana cerana. Within this study, an OBP gene (AccOBP10) was isolated and identified from Apis cerana cerana. Both homology and phylogenetic relationship analyses indicated that the amino acid sequence of AccOBP10 had a high degree of sequence identity with other members of the gene family. Analysis of real-time quantitative PCR (qRT-PCR) showed that AccOBP10 mRNA was expressed at higher levels in the venom gland than in other tissues. The mRNA transcript expression of AccOBP10 was upregulated by low temperature (4 °C), hydrogen peroxide (H₂O₂), pyridaben, methomyl and imidacloprid but downregulated by heat (42 °C), ultraviolet (UV) light, vitamin C, mercuric chloride (HgCl₂), cadmium chloride (CdCl₂), paraquat and phoxim. Expression of AccOBP10 under abiotic stress was analyzed by Western blotting, and the results were consistent with those of qRT-PCR. And as a further study of AccOBP10 function, we demonstrated that knockdown of AccOBP10 by RNA interference (RNAi) could slightly increase the expression levels of some stress-related genes. Collectively, these results suggest that AccOBP10 is mainly involved in the response to stress conditions.

中文翻译：

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中华蜜蜂新气味结合蛋白基因AccOBP10的分子和功能表征。

气味结合蛋白（OBP）在昆虫的气味感知和运输中起重要作用。然而，知之甚少OBPs是否在昆虫执行其他功能，尤其是在中华蜜蜂。在这项研究中，从蜜蜂Apis cerana cerana分离并鉴定了OBP基因（AccOBP10）。同源性和系统发育关系分析均表明，AccOBP10的氨基酸序列与该基因家族的其他成员具有高度的序列同一性。实时定量PCR（qRT-PCR）分析表明，AccOBP10 mRNA在毒腺中的表达水平高于其他组织。AccOBP10的mRNA转录本表达在低温（4°C），过氧化氢（H ₂ O ₂），哒嗪，灭多威和吡虫啉中被上调，但在热（42°C），紫外线（UV），维生素C，氯化汞（HgCl ₂）下调，氯化镉（CdCl ₂），百草枯和辛硫磷。通过蛋白质印迹分析了AbOBP10在非生物胁迫下的表达，其结果与qRT-PCR一致。作为对AccOBP10功能的进一步研究，我们证明了RNA干扰（RNAi）敲除AccOBP10可能会稍微增加一些与压力相关的基因的表达水平。总体而言，这些结果表明AccOBP10主要参与对压力条件的响应。