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Chemo-Radiative Stress of Plasma as a Modulator of Charge-Dependent Nanodiamond Cytotoxicity
ACS Applied Bio Materials ( IF 4.7 ) Pub Date : 2020-09-14 , DOI: 10.1021/acsabm.0c01000
Peiyu Wang 1, 2, 3 , Rusen Zhou 4 , Renwu Zhou 1, 4, 5 , Nina Recek 4, 6 , Karthika Prasad 4 , Robert Speight 4 , Derek Richard 1, 2, 3 , Patrick J Cullen 5 , Erik W Thompson 1, 2, 3 , Kostya Ken Ostrikov 1, 4 , Kateryna Bazaka 4, 7
Affiliation  

Efficient and selective internalization of nanoscale diamonds (also termed nanodiamonds, NDs) by living cells is of fundamental importance for their bionanotechnological applications. The biocompatibility of NDs is well established and has been suggested to arise from the limited membrane perturbation during their cellular translocation. However, the latter may be affected when cells are subjected to external stress. This study shows that the oxidative stress generated by atmospheric pressure cold plasmas (APCP) alters cell sensitivity to NDs, and their cytotoxicity profile. Both positively and negatively charged NDs are nontoxic to cells, here Saccharomyces cerevisiae and human cell lines, i.e., near-normal human mammary epithelial cells (MCF-10A) and breast cancer cells (MDA-MB-468 and T47D), unless the APCP stress is introduced. A brief exposure of the cells to APCP leads to a significant increase in their ND affinity (uptake and/or surface attachment) and intracellular ROS accumulation, particularly for positively charged NDs and both yeast and cancer cells. A concomitant decrease in cell viability and yeast cell growth, reflected by longer lag phases and lower cell density after 24 h of incubation, demonstrates a considerably enhanced ND toxicity to these cells. These results suggest that chemo-radiative stress, such as that produced by plasma, may influence the toxicity of nanoparticles to different cells, with specificity achieved through controlling particle charges. Moreover, since oxidative stress is not only associated with the use of APCP but can arise unintentionally within an organism and/or in the environment, these findings may have broader implications for the use of nontoxic nanoparticles in bionanotechnology in general.

中文翻译:

等离子体的化学辐射应激作为电荷依赖性纳米金刚石细胞毒性的调节剂

活细胞对纳米级金刚石(也称为纳米金刚石,ND)的有效和选择性内化对其生物纳米技术应用至关重要。ND 的生物相容性已得到充分确立,并且已被认为是由于其细胞易位过程中有限的膜扰动引起的。但是,当细胞受到外部压力时,后者可能会受到影响。这项研究表明,大气压冷等离子体 (APCP) 产生的氧化应激会改变细胞对 ND 的敏感性及其细胞毒性特征。带正电荷和带负电荷的 ND 对细胞无毒,此处为酿酒酵母和人类细胞系,.,接近正常的人类乳腺上皮细胞 (MCF-10A) 和乳腺癌细胞 (MDA-MB-468 和 T47D),除非引入 APCP 应激。将细胞短暂暴露于 APCP 会导致其 ND 亲和力(摄取和/或表面附着)和细胞内 ROS 积累显着增加,特别是对于带正电荷的 ND 以及酵母和癌细胞。细胞活力和酵母细胞生长同时下降,反映在孵育 24 小时后较长的滞后期和较低的细胞密度,表明 ND 对这些细胞的毒性显着增强。这些结果表明,化学辐射应力,例如由等离子体产生的应力,可能会影响纳米粒子对不同细胞的毒性,通过控制粒子电荷来实现特异性。而且,
更新日期:2020-10-21
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