ABSTRACT

As a chronic inflammatory disease, coronary artery disease (CAD) is a common cause of death worldwide. Dysregulation of microRNA expression levels in peripheral blood mononuclear cells (PBMCs) may contribute to CAD and serve as a potential diagnostic biomarker. Here, we evaluated PBMC expression of two CAD-related inflammatory miRNAs, miR-196a and miR-100, in PBMCs of CAD patients with significant stenosis (CAD, n: 72), patients with insignificant coronary stenosis (ICAD, n: 30), and controls (n: 74) and checked whether they can segregate study groups. MiRNA expression was evaluated using the standard stem-loop RT-qPCR method. MiR-196a expression was downregulated in ICAD compared to CADs and healthy groups. MiR100 expression levels were not different between groups. The receiver operating characteristic (ROC) curve analysis acquainted that miR-196a expression levels in PBMC could segregate CAD individuals or any of its clinical manifestations (i.e. unstable angina, stable angina, acute myocardial infarction) from ICADs. In conclusion, this study reported a distinct miR-196a expression pattern in PBMCs of all patient groups and recommended a biomarker potential for miR-196a in discriminating ICADs from CADs or healthy controls.

摘要

作为一种慢性炎症性疾病，冠状动脉疾病（CAD）是全球常见的死亡原因。外周血单个核细胞 (PBMC) 中 microRNA 表达水平的失调可能导致 CAD 并作为潜在的诊断生物标志物。在这里，我们评估了两种 CAD 相关炎症性 miRNA，miR-196a 和 miR-100，在具有显着狭窄的 CAD 患者 (CAD，n: 72)、具有不显着冠状动脉狭窄的患者 (ICAD, n: 30) 的 PBMC 中的 PBMC 表达, 和对照组 (n: 74) 并检查他们是否可以隔离研究组。使用标准茎环 RT-qPCR 方法评估 miRNA 表达。与 CAD 和健康组相比，ICAD 中 MiR-196a 的表达下调。MiR100 表达水平在各组之间没有差异。受试者工作特征（ROC）曲线分析表明，PBMC 中的 miR-196a 表达水平可以将 CAD 个体或其任何临床表现（即不稳定型心绞痛、稳定型心绞痛、急性心肌梗塞）与 ICAD 区分开来。总之，本研究报告了所有患者组的 PBMC 中独特的 miR-196a 表达模式，并推荐了 miR-196a 在区分 ICAD 与 CAD 或健康对照方面的生物标志物潜力。