Key Points Rectal balloon collections minimize blood contamination of mucosal Ig. Rectal balloon collections require ring lubrication to reduce blood contamination. Rectal balloon collection is quick and well tolerated in humans. Measurements of IgG and IgA in human rectal secretions are used to evaluate the Abs elicited by HIV vaccines or the bioaccumulation following immunoprophylaxis at the sites of HIV exposure. To improve sampling methods and tolerability of the procedure, we optimized a balloon device (OriCol) for rectal microbiome sampling requiring 10 second inflation and compared this method to a 5 minute collection using sponges. Lubrication of the device did not interfere with IgG, IgA, or hemoglobin ELISA. Lubricated OriCols inflated to 30 cc minimized hemoglobin contamination (<4.68 ng/ml) compared with collections with two sponge types (Weck-Cel: 267.2 ng/ml, p < 0.0001; and Merocel: 59.38 ng/ml, p = 0.003). Median human serum albumin for OriCols was 14.9 μg/ml, whereas Merocels and Weck-Cels were 28.57 μg/ml (p = 0.0005) and 106.2 μg/ml (p = 0.0002), respectively. Consistent with reduced systemic contamination, the median IgG measured in OriCol-collected rectal secretions (986 ng) was lower than secretions from sponges (Weck-Cel: 8588 ng, p < 0.0001; Merocel: 2509 ng, p = 0.0389). The median IgA yield of samples using the OriCol method (75,253 ng) was comparable to that using Merocel (71,672 ng; p = 0.6942) but significantly higher than Weck-Cel sponges (16,173 ng, p = 0.0336). Median recovery volumes for OriCols were 800 μl, whereas Merocels and Weck-Cels were 615 μl (p = 0.0010) and 655 μl (p = 0.0113), respectively. The balloon device was acceptable among 23 participants, as 85.1% experiencing their first collection ranked it as “seven: acceptable – a lot” or “six: acceptable – somewhat” in a seven-point Likert scale. Therefore, lubricated OriCols inflated to 30 cc allowed for a rapid, well-tolerated, blood-free collection of human rectal secretions.

中文翻译：

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使用 OriCol 设备快速收集人类直肠分泌物适用于测量无血液污染的粘膜 Ig

要点 直肠球囊收集可最大限度地减少粘膜 Ig 的血液污染。直肠球囊采集需要环润滑以减少血液污染。直肠气球收集在人类中快速且耐受性良好。人类直肠分泌物中 IgG 和 IgA 的测量用于评估 HIV 疫苗引起的 Abs 或在 HIV 暴露部位进行免疫预防后的生物蓄积。为了改进取样方法和程序的耐受性，我们优化了用于需要 10 秒充气的直肠微生物组取样的球囊装置 (OriCol)，并将该方法与使用海绵进行 5 分钟收集的方法进行了比较。设备的润滑不会干扰 IgG、IgA 或血红蛋白 ELISA。润滑的 OriCols 充气至 30 cc，最大限度地减少了血红蛋白污染（<4. 68 ng/ml) 与两种海绵类型的集合相比（Weck-Cel：267.2 ng/ml，p < 0.0001；和 Merocel：59.38 ng/ml，p = 0.003）。OriCols 的人血清白蛋白中位数为 14.9 μg/ml，而 Merocels 和 Weck-Cels 分别为 28.57 μg/ml (p = 0.0005) 和 106.2 μg/ml (p = 0.0002)。与全身污染减少一致，在 OriCol 收集的直肠分泌物中测得的中位 IgG (986 ng) 低于海绵分泌物 (Weck-Cel：8588 ng，p < 0.0001；Merocel：2509 ng，p = 0.0389)。使用 OriCol 方法的样品 IgA 产量中位数 (75,253 ng) 与使用 Merocel 的样品 (71,672 ng; p = 0.6942) 相当，但明显高于 Weck-Cel 海绵 (16,173 ng, p = 0.0336)。OriCols 的中位回收体积为 800 μl，而 Merocels 和 Weck-Cels 为 615 μl (p = 0.0010) 和 655 μl (p = 0.0113)，分别。气球装置在 23 名参与者中是可以接受的，因为 85.1% 的人在第一次收集时将其评为“7：可接受 - 很多”或“6：可接受 - 有点”，采用七分李克特量表。因此，润滑的 OriCols 充气到 30 cc 可以快速、耐受性良好、无血地收集人类直肠分泌物。