The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin‐resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin‐treated and nontreated L. striatellus was performed to screen the buprofezin‐specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin‐treated L. striatellus, RNAi‐based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration‐dependent manner. In addition, a cocktail of double‐stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi‐based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application.

中文翻译：

---

靶向丁苯丙酸特异性基因的dsRNA对小褐飞虱Laodelphax striatellus的杀虫和协同活性。

小褐飞虱，Laodelphax striatellus，是稻田中的一种有害害虫。尽管已将buprofezin用于控制纹状体乳杆菌已有十多年的历史，但 最近已报道了对buprofezin耐药的L. striatellus的发生。为了开发一种替代性的害虫控制策略，进行了丁苯丙酸处理和未处理的L. striatellus的对比转录组分析，以筛选用于RNA干扰（RNAi）应用的丁苯丙酸特异性靶基因。其中在噻嗪酮处理的下调六种基因灰飞虱，RNAi的基于lipophorin前体，endocuticle结构的糖蛋白的沉默，和几丁质合成酶显著诱导的致死率纹状体乳杆菌以浓度依赖性方式存在。此外，针对这三个基因的双链RNA混合物显示出与丁丙哌嗪的协同作用。这些结果提供了基于RNAi的控制纹状体乳杆菌的有效方法，以及为RNAi应用鉴定新型靶基因的有效方法。