The effects of dietary poly‐β‐hydroxybutyrate (PHB) on the lipid and fatty acids (FA) in crustaceans were investigated using Artemia as model species. Supplying PHB either in crystalline or amorphous form significantly increased the whole‐body lipid contents of starved Artemia. Co‐supplying dietary PHB with bacterial PHB degrader Comamonas testosteroni at 10⁶ cells ml⁻¹ for 24 hr also significantly increased this parameter. These findings have important implications on the lipid‐saving effects of PHB in crustacean tissues. Dietary PHB, however, did not contribute to the increase in docosahexaenoic acid (DHA) of the naturally DHA‐deprived Artemia. Alternative strategy of co‐supplying dietary PHB with highly unsaturated fatty acid (HUFA)‐rich emulsion for 24 hr effectively improved the nutritional contents of Artemia and at the same time assured efficient delivery of PHB to the larval predator. According to a ¹³C stable isotope tracer study, the significantly higher δ¹³C levels measured in Artemia 2 hr postfeeding with ¹³C‐labelled R. eutropha DSM545 indicate that PHB offered in natural matrix is rapidly assimilated. Overall, this study demonstrated the lipid‐saving effects of PHB in Artemia. PHB assimilation following gastrointestinal degradation could attribute to its reported beneficial effects in various aquatic farmed species.

中文翻译：

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甲壳动物模型有机体Artemia sp。中的脂质和脂肪酸组成。受补充聚-β-羟基丁酸酯（PHB）的影响

以Artemia为模型物种，研究了饮食中的聚β-羟基丁酸酯（PHB）对甲壳类动物的脂质和脂肪酸（FA）的影响。以结晶或无定形形式提供PHB会显着增加饥饿的卤虫的全身脂质含量。膳食PHB与细菌PHB降解物睾丸激素Comamonas testosteroni的共供应量为10 ^6个细胞ml ^-1，持续24小时也大大提高了该参数。这些发现对甲壳动物组织中PHB的节脂作用具有重要意义。饮食中的PHB并不能促进自然DHA剥夺性贫血中二十二碳六烯酸（DHA）的增加。膳食PHB与富含高度不饱和脂肪酸（HUFA）的乳剂共同供应24小时的替代策略可有效改善卤虫的营养含量，同时确保将PHB有效地递送至幼虫捕食者。根据本发明的¹³ Ç稳定同位素示踪研究中，显著更高δ ^13个中测量C水平卤虫用2小时postfeeding ¹³ C标记的富氧罗尔斯通氏DSM545表明PHB在天然基质提供迅速吸收。总体而言，这项研究证明了PHB在卤虫病中的节脂作用。胃肠道降解后的PHB吸收可能归因于其在各种水产养殖物种中的有益作用。