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Bioassay-guided isolation of two antifungal compounds from Magnolia officinalis, and the mechanism of action of honokiol
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.pestbp.2020.104705
Yin-Fang Yan , Cheng-Jie Yang , Xiao-Fei Shang , Zhong-Min Zhao , Ying-Qian Liu , Rui Zhou , Hua Liu , Tian-Lin Wu , Wen-Bin Zhao , Yu-Ling Wang , Guan-Fang Hu , Fang Qin , Ying-Hui He , Hai-Xin Li , Sha-Sha Du

Magnolia officinalis, as a well-known herb worldwide, has been widely used to treat multiple diseases for a long time. In this study, the petroleum ether extract from M. officinalis showed effective antifungal activity against seven plant pathogens (particularly against R. solani with an inhibition rate of 100.00% at 250 μg/mL). Honokiol and magnolol, isolated by the bioassay-guided method, exhibited greater antifungal activity than tebuconazole (EC50 = 3.07 μg/mL, p ≤ 0.001) against R. solani, which EC50 values were 2.18 μg/mL and 3.48 μg/mL, respectively. We used transcriptomics to explore the mechanism of action of honokiol against R. solani. Results indicated that honokiol may exert antifungal effects by blocking the oxidative phosphorylation metabolic pathway. Further studies indicated that honokiol induced ROS overproduction, disrupted the mitochondrial function, affected respiration, and blocked the TCA cycle, which eventually inhibited ATP production. Besides, honokiol also damaged cell membranes and caused morphological changes. This study demonstrated that the lignans isolated from M. officinalis possess the potential to be developed as botanical fungicides.

中文翻译:

从厚朴中生物测定引导分离两种抗真菌化合物,以及和厚朴酚的作用机制

厚朴作为世界著名的草药,长期以来被广泛用于治疗多种疾病。在本研究中,厚朴的石油醚提取物对七种植物病原体(特别是对茄病菌的抑制率为 100.00%,浓度为 250 μg/mL)显示出有效的抗真菌活性。通过生物测定指导方法分离的和厚朴酚和厚朴酚对 R. solani 表现出比戊唑醇(EC50 = 3.07 μg/mL,p ≤ 0.001)更大的抗真菌活性,其 EC50 值分别为 2.18 μg/mL 和 3.48 μg/mL . 我们使用转录组学来探索和厚朴酚对 R. solani 的作用机制。结果表明和厚朴酚可能通过阻断氧化磷酸化代谢途径发挥抗真菌作用。进一步的研究表明,和厚朴酚会导致 ROS 过量产生,扰乱线粒体功能,影响呼吸,阻断 TCA 循环,最终抑制 ATP 的产生。此外,和厚朴酚还会破坏细胞膜并引起形态变化。该研究表明,从厚朴中分离的木脂素具有作为植物杀菌剂开发的潜力。
更新日期:2020-11-01
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