A fluorescence assay based on paraoxonase (PO) and laccase (Lac) immobilized on anthracene-sequestered polyamic acid films is now being reported for the first time for ciprofloxacin (CFx) detection. In enzymatic reaction, PO converts phenyl acetate (PA) to acetic acid and phenol. The formed phenol is further oxidized by laccase using oxygen as a co-substrate. For indirectly fluorescence measurements of PA hydrolysis, increased fluorescence intensity was measured after oxygen consumption by laccase. This is because oxygen is quencher of anthracene (Ant) incorporated in the sequestered poly(amic) acid (PAA) film. The detection mechanism was based on inhibition of PO activity by CFx. Using PAA provided the advantage of controlling the film thickness. Firstly, morphology of PAA-Ant polymeric film was characterized by scanning electron microcopy (SEM) and the success of PO-Lac immobilization on PAA-Ant was proven by SEM plus Energy-dispersive X-ray spectroscopy (SEM-EDX) and fluorescence measurements. Then Ant-PAA/PO/Lac is calibrated for PA and CFx without any interfering of some potential interferences. All results make it a promising tool for monitoring CFx at minute levels in samples.

现在首次报道了基于对氧磷酶（PO）和漆酶（Lac）固定在蒽异氰酸酯化聚酰胺酸薄膜上的荧光检测法用于环丙沙星（CFx）的检测。在酶促反应中，PO将乙酸苯酯（PA）转化为乙酸和苯酚。使用氧气作为共底物，形成的苯酚被漆酶进一步氧化。对于PA水解的间接荧光测量，漆酶耗氧后测量了增加的荧光强度。这是因为氧是结合在螯合聚（酰胺）酸（PAA）膜中的蒽（Ant）的猝灭剂。检测机制基于CFx抑制PO活性。使用PAA提供了控制薄膜厚度的优势。首先，通过扫描电子显微镜（SEM）表征了PAA-Ant聚合物薄膜的形貌，并通过SEM加能量色散X射线光谱（SEM-EDX）和荧光测量证明了PO-Lac在PAA-Ant上的固定成功。然后，针对PA和CFx校准Ant-PAA / PO / Lac，而不会干扰任何潜在的干扰。所有结果使它成为监测微量样品中CFx的有前途的工具。