Abstract The risk of catching brucellosis and the absence of effective human vaccines has led to the use of new strategies to produce new vaccines against infections caused by this bacterium. In this study, the effect of two new designed nanovaccines on the production of immunoglobulins and their proper functions was investigated. Firstly, the production of immunoglobulins in the serum of the mouse model after three vaccination periods with lipopolysaccharide and oligopolysaccharide antigens in conjugation with PLGA nanoparticles were studied. The mice were divided into 6 groups and each group received LPS-PLGA, OPS-PLGA, LPS, OPS, PLGA and PBS separately. Immunoglobulin titers were measured every two weeks after each vaccination period. Two weeks after the last vaccination, opsonophagocytosis test were performed to evaluate the efficacy and function of produced immunoglobulins. In order to investigate the protective effect of nanovaccines in vivo, the mice were challenged with Brucella abortus bacteria. Immune responses have increased on immunoglobulin production after each vaccination period. The results of the opsonophagocytosis assay showed that produced immunoglobulins have the superior performance to antibodies produced against the pure antigens. By performing the challenge assay, it was found that the immune system of mice receiving new nanovaccines were able to remove more bacteria from the body of mice. In general, it can be concluded that both new prepared nanovaccines can be considered to improve the immunization process in animals and humans against brucellosis although LPS-PLGA conjugate creates more immunogenicity compared to OPS-PLGA nanovaccine.

中文翻译：

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基于与 PLGA 纳米粒子偶联的 OPS 和 LPS 抗原的两种新型纳米疫苗对布鲁氏菌的免疫作用

摘要 感染布鲁氏菌病的风险和缺乏有效的人类疫苗促使人们使用新策略来生产针对这种细菌引起的感染的新疫苗。在这项研究中，研究了两种新设计的纳米疫苗对免疫球蛋白产生及其适当功能的影响。首先，研究了用脂多糖和寡多糖抗原与 PLGA 纳米颗粒共轭的三个疫苗接种期后小鼠模型血清中免疫球蛋白的产生。将小鼠分为6组，每组分别接受LPS-PLGA、OPS-PLGA、LPS、OPS、PLGA和PBS。在每个疫苗接种期后每两周测量免疫球蛋白滴度。在最后一次接种疫苗两周后，进行调理吞噬试验以评估产生的免疫球蛋白的功效和功能。为了研究纳米疫苗在体内的保护作用，小鼠接受了流产布鲁氏菌的攻击。每个疫苗接种期后，免疫反应对免疫球蛋白的产生都有所增加。调理吞噬试验的结果表明，产生的免疫球蛋白比针对纯抗原产生的抗体具有更好的性能。通过进行攻击试验，发现接受新纳米疫苗的小鼠的免疫系统能够从小鼠体内清除更多细菌。一般来说，