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New perspective to improve dentin-adhesive interface stability by using dimethyl sulfoxide wet-bonding and epigallocatechin-3-gallate.
Dental Materials ( IF 5 ) Pub Date : 2020-09-14 , DOI: 10.1016/j.dental.2020.08.009
Zhongni Zhang 1 , Jian Yu 1 , Chenmin Yao 1 , Hongye Yang 1 , Cui Huang 1
Affiliation  

Objectives

To determine whether dentin–adhesive interface stability would be improved by dimethyl sulfoxide (DMSO) wet-bonding and epigallocatechin-3-gallate (EGCG).

Methods

Etched dentin surfaces from sound third molars were randomly assigned to five groups according to different pretreatments: group 1, water wet-bonding (WWB); group 2, 50% (v/v) DMSO wet-bonding (DWB); groups 3–5, 0.01, 0.1, and 1 wt% EGCG-incorporated 50% (v/v) DMSO wet-bonding (0.01%, 0.1%, and 1%EGCG/DWB). Singlebond universal adhesive was applied to the pretreated dentin surfaces, and composite buildups were constructed. Microtensile bond strength (μTBS) and interfacial nanoleakage were respectively examined after 24 h water storage or 1-month collagenase ageing. In situ zymography andStreptococcus mutans (S. mutans) biofilm formation were also investigated.

Results

After collagenase ageing, μTBS of groups 4 (0.1%EGCG/DWB) and 5 (1%EGCG/DWB) did not decrease (p > 0.05) and was higher than that of the other three groups (p < 0.05). Nanoleakage expression of groups 4 and 5 was less than that of the other three groups (p < 0.05), regardless of collagenase ageing. Metalloproteinase activities within the hybrid layer in groups 4 and 5 were suppressed. Furthermore, pretreatment with 1%EGCG/DWB (group 5) efficiently inhibited S. mutans biofilm formation along the dentin–adhesive interface.

Significance

This study suggested that the synergistic action of DMSO wet-bonding and EGCG can effectively improve dentin–adhesive interface stability. This strategy provides clinicians with promising benefits to achieve desirable dentin bonding performance and to prevent secondary caries, thereby extending the longevity of adhesive restorations.



中文翻译:

通过使用二甲基亚砜湿粘合和表没食子儿茶素-3-没食子酸酯提高牙本质-粘合剂界面稳定性的新视角。

目标

确定二甲基亚砜 (DMSO) 湿粘合和表没食子儿茶素-3-没食子酸酯 (EGCG) 是否会改善牙本质-粘合剂界面稳定性。

方法

根据不同的预处理,将健全的第三磨牙的蚀刻牙本质表面随机分为五组:第一组,水湿粘合(WWB);第 2 组,50% (v/v) DMSO 湿粘合 (DWB);第 3-5 组、0.01、0.1 和 1 wt% EGCG 并入 50% (v/v) DMSO 湿粘合(0.01%、0.1% 和 1%EGCG/DWB)。将单键通用粘合剂应用于预处理的牙本质表面,并构建复合材料。分别在 24 h 水储存或 1 个月胶原酶老化后检测微拉伸粘合强度 (μTBS) 和界面纳米泄漏。还研究了原位酶谱和变形链球菌( S. mutans ) 生物膜的形成。

结果

胶原酶老化后,第4组(0.1%EGCG/DWB)和第5组(1%EGCG/DWB)的μTBS没有下降(p >0.05),高于其他三组(p <0.05)。与胶原酶老化无关,第4 组和第 5 组的纳米渗漏表达低于其他三组(p < 0.05)。第 4 组和第 5 组杂合层内的金属蛋白酶活性受到抑制。此外,用 1%EGCG/DWB(第 5 组)预处理有效地抑制了沿牙本质-粘合剂界面形成的变形链球菌生物膜。

意义

该研究表明,DMSO湿粘合和EGCG的协同作用可以有效提高牙本质-粘合剂界面的稳定性。这种策略为临床医生提供了有希望的好处,以实现理想的牙本质粘合性能并防止继发性龋齿,从而延长粘合修复的寿命。

更新日期:2020-10-30
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