Acid mine drainage (AMD) is one of an important pollution sources associated with mining activities and often inhibits plant growth. Plant growth promoting bacteria has received extensive attention for enhancing adaptability of plants growing in AMD polluted soils. The present study investigated the effect of plant growth promoting Bacillus spp. (strains UM5, UM10, UM13, UM15 and UM20) to improve vetiver (Chrysopogon zizanioides L.) adaptability in a soil irrigated with 50% AMD. Bacillus spp. exhibited P-solubilization, IAA and siderophore production. The Bacillus spp. strains UM10 and UM13 significantly increased shoot (4.2-2.5%) and root (3.4-1.9%) biomass in normal and AMD-impacted soil, respectively. Bacillus sp. strain UM20 significantly increased soil AP (379.93 mg/kg) while strain UM13 increased TN (1501.69 mg/kg) and WEON (114.44 mg/kg) than control. Proteobacteria, Chloroflexi, Acidobacteria and Bacteroidetes are the dominant phyla, of which Acidobacteria (12%) and Bacteroidetes (8.5%) were dominated in soil inoculated with Bacillus sp. strain UM20 while Proteobacteria (70%) in AMD soil only. However, the Chao1 and evenness indices were significantly increased in soil inoculated with Bacillus sp. strain UM13. Soil pH, AP and N fractions were positively correlated with the inoculation of bacterial strains UM13 and UM20. Plant growth promoting Bacillus spp. strains UM13 and UM20 were the main contributors to the variations in the rhizosphere bacterial community structure, improving soil available P, TN, WEON, NO₃^--N thus could be a best option to promote C. zizanioides adaptability in AMD-impacted soils.

酸性矿山排水（AMD）是与采矿活动相关的重要污染源之一，通常会抑制植物的生长。促进植物生长的细菌已经受到广泛关注，以提高在AMD污染土壤中生长的植物的适应性。本实验研究植物生长促进效应芽孢杆菌属。（品系UM5，UM10，UM13，UM15和UM20）可提高香根草（Chrysopogon zizanioides L.）在50％AMD灌溉的土壤中的适应性。芽孢杆菌属。表现出P增溶，IAA和铁载体的产生。该芽孢杆菌属。在正常和受AMD影响的土壤中，菌株UM10和UM13分别显着增加了芽（4.2-2.5％）和根（3.4-1.9％）的生物量。芽孢杆菌属。与对照相比，UM20菌株显着增加了土壤AP（379.93 mg / kg），而UM13菌株则增加了TN（1501.69 mg / kg）和WEON（114.44 mg / kg）。变形菌，绿弯菌门，酸杆菌门和拟杆菌是主要的类群，其中酸杆菌门（12％）和拟杆菌（8.5％）在土壤中接种物为主芽孢杆菌属。菌株UM20，而变形杆菌（70％）仅在AMD土壤中。但是，接种芽孢杆菌的土壤中，Chao1和均匀度指数显着增加sp。菌株UM13。土壤pH，AP和N分数与细菌菌株UM13和UM20的接种呈正相关。促进芽孢杆菌属的植物生长。UM13和UM20菌株是根际细菌群落结构变化的主要贡献者，提高土壤有效磷，TN，WEON，NO ₃ ^-- N可能因此是促进C. zizanioides在受AMD影响的土壤中适应性的最佳选择。