Nitric oxide (NO)-based gas therapy is emerged as a new adjunct anti-tumor treatment method, which has triggered a great research interest. Nevertheless, due to the short half-life oBalbf NO gas in vivo, it is of significance to develop NO-gas based gasotransmitter with controllable NO release for deep-tissue anti-tumor therapy. Herein, a novel soft X-ray activated persistent luminescence nanotransducer is designed for controllable and long-lasting NO release and deep-tissue anti-cancer therapy by integrating ZnGa₂O₄:Mn (ZGO:Mn) nanoparticles with light-responsive NO donor (RBS). With the merits of the ultra-low dosage (down to 0.90 mGy) soft X-ray activated persistent luminescence from ultra-small sized ZGO:Mn, continuous NO release is achieved for about 40 min after stopping the irradiation of X-ray. Moreover, the green persistent luminescence can be renewably activated by in-situ soft X-ray irradiation, leading to the repeatable long-lasting NO release in deep tissue (up to 24 mm). And the designed NO-releasing platform presents efficient in vitro and in vivo anti-cancer therapy. Therefore, the designed persistent luminescence-based NO gasotransmitter provides a new NO-releasing strategy for depth-independent gas-sensitized therapeutic applications.

基于一氧化氮（NO）的气体疗法已成为一种新的辅助抗肿瘤治疗方法，引起了极大的研究兴趣。然而，由于体内oBalbf NO气体的半衰期短，因此开发具有可控NO释放量的NO气体基气体递质对于深层组织抗肿瘤治疗具有重要意义。本文中，通过整合ZnGa ₂ O _4，设计了一种新型的软X射线持续发光纳米换能器，用于可控和持久的NO释放和深层组织抗癌治疗。：Mn（ZGO：Mn）纳米颗粒，具有光响应性NO供体（RBS）。凭借超低剂量（低至0.90 mGy）的优点，软X射线激活了来自超小型ZGO：Mn的持续发光，在停止X射线照射后约40分钟即可连续释放NO。此外，可以通过原位软X射线辐射来激活绿色持续发光，从而在深部组织（最大24毫米）中产生可重复的持久性NO释放。设计的NO释放平台可提供有效的体外和体内抗癌治疗。因此，设计的基于持久发光的NO气体发射器为与深度无关的气体敏化治疗应用提供了新的NO释放策略。