The accumulation of advanced glycation end-products (AGEs) correlates with aging and accompanies the onset of age-related diseases, such as diabetes and arteriosclerosis. Therefore, a daily intake of natural compounds that inhibit the production of AGEs may be beneficial in preventing these diseases. In this study, we evaluated the inhibitory effects of 14 natural crude extracts, including those of Drosera species, which possess anti-inflammatory activity, on the formation of AGEs, such as N^ω-(carboxymethyl)arginine (CMA) and N^ε-(carboxymethyl)lysine (CML). Crude extracts of Drosera inhibited the formation of CMA and CML by incubation on gelatin with ribose more effectively than with other extracts, so active compounds that prevent AGE formation were purified from Drosera tokaiensis, which is endemic to Japan. Several compounds were purified from D. tokaiensis extracts using HPLC and identified by NMR analysis. These compounds included ellagic acid, 3,3′-di-O-methylellagic acid 4′-glucoside, myricitrine, and quercimelin. Furthermore, all compounds showed a significantly higher inhibitory effect on CMA and CML formations than aminoguanidine. Specifically, ellagic acid and myricitrine had the highest inhibitory effects of the compounds tested. However, not all compounds showed inhibition of CMA formation in a mixture of gelatin and glyoxal (GO). These results suggest that the compounds in D. tokaiensis inhibit CMA and CML formations via the antioxidative activity of phenolic compounds, rather than GO trapping action. This study provides the first evidence that D. tokaiensis inhibits CMA and CML formations and that phenolic compounds such as ellagic acid and myricitrine play an important role as active components of D. tokaiensis extracts.

晚期糖基化终产物（AGEs）的积累与衰老相关，并伴随着与年龄有关的疾病（如糖尿病和动脉硬化）的发作。因此，每天摄入抑制AGEs产生的天然化合物可能有益于预防这些疾病。在这项研究中，我们评估的14种天然的粗提取物，包括那些的抑制作用茅膏菜物种，其具有抗炎活性，对终产物，如形成Ñ ^ω - （羧甲基）精氨酸（CMA）和Ñ ^ε - （羧甲基）赖氨酸（CML）。茅膏菜的粗提物通过与其他提取物一起在明胶中与核糖一起孵育，可更有效地抑制CMA和CML的形成，因此从日本特有种Drosera tokaiensis中纯化了可防止AGE形成的活性化合物。使用HPLC从东海杜鹃提取物中纯化了几种化合物，并通过NMR分析鉴定。这些化合物包括鞣花酸3,3'-di- O-甲基鞣花酸4'-葡萄糖苷，杨梅碱和槲皮素。此外，所有化合物对CMA和CML形成的抑制作用均比氨基胍显着更高。具体而言，鞣花酸和肉豆蔻碱具有所测试化合物的最高抑制作用。但是，并非所有化合物在明胶和乙二醛（GO）的混合物中均显示出对CMA形成的抑制作用。这些结果表明，D。tokaiensis中的化合物通过酚类化合物的抗氧化活性而不是GO捕集作用来抑制CMA和CML的形成。这项研究提供了第一个证据，即海藻D.tokaiensis抑制了CMA和CML的形成，酚醛化合物如鞣花酸和肉豆蔻碱起着重要的作用。D. tokaiensis提取物。