Abstract

The feasibility of applying higher plant biotechnology methods and approaches to conservation and propagation of Lilium pensylvanicum is demonstrated. Using seeds as the primary explants enables one to introduce this species into an in vitro culture while not causing harm to mother plants. Subculturing aseptic bulbs on a Humburg medium with halved composition of salts of macro- and microelements and enriched with kinetin (5 mg/L) and indoleacetic acid (1 mg/L) gives rise to the formation of 3 ± 1 bulbs per explant. Subsequent passages on four different variants of culture medium identifies the Murashige and Skoog medium supplemented with 6-benzylaminopurine (0.4 mg/L) as the most suitable for promoting the bulb development and offshoot growth. Rooting is carried out on hormone-free media. Years-long observations over the acclimatization of ex situ regenerated plants have been performed for the first time. The 5-year-long field observations over the survival, growth, and development of regenerated plants (n = 30) under the conditions of the collection nursery of Yakutsk Botanical Garden show that 77% of specimens have been acclimatized successfully. Mass flowering and fruiting is observed to take place in the fourth year. The identified ontogenetic developmental features of young plants are the morphological polyvariety of flowers and a high variability of the stem height. The developed protocol of clonal micropropagation of Lilium pensylvanicum, along with the results of many years of observations over the growth of regenerated plants, suggest that this method can be recommended for propagation of declining cenopopulations.

中文翻译：

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稀有种Lilium pensylvanicum Ker.-Gawl的体外繁殖以及非原位生长和发育。（紫ilia科）

摘要

应用高等植物生物技术方法和方法进行戊烯百合的保存和繁殖的可行性被证明。使用种子作为主要外植体可以使该物种引入体外培养，同时又不会对母本植物造成伤害。将腐殖质球茎在Humburg培养基上亚种，其中的宏观元素和微量元素的盐减半，并富含激动素（5 mg / L）和吲哚乙酸（1 mg / L），每个外植体形成3±1个球茎。随后在四种不同的培养基变体上传代，鉴定出补充有6-苄基氨基嘌呤（0.4 mg / L）的Murashige和Skoog培养基最适合促进球茎发育和分支生长。生根在无激素的培养基上进行。关于异地再生植物适应化的长达数年的观察是首次进行。对生存，生长，n = 30）在雅库茨克植物园的收集苗圃的条件下，表明77％的标本已成功适应环境。观察到第四年大量开花结果。已确定的年幼植物的个体发育发育特征是花的形态多样性和茎高的高度变异性。无性系快繁发达协议毛百合，具有多年在再生植物的生长观测的结果一起，建议可推荐用于cenopopulations下降的传播这种方法。