The earthworm (Drawida gisti) is an ecologically important sentinel species for soils that is widely distributed throughout Eastern Asia; however, the molecular tools required for genetic diversity studies of this earthworm are still rare. The aim of the study was to develop and characterize microsatellite markers in D. gisti and to evaluate their transferability to other Drawida species. We employed a RAD-seq approach to develop 12 microsatellite markers for D. gisti. The characterization and analysis of loci was achieved using 24 individuals of D. gisti from a natural population. The number of alleles per locus ranged from four to eleven, with an average of 6.5. Observed and expected heterozygosities varied from 0.708 to 0.958, and from 0.568 to 0.883, respectively. No loci presented significant deviations from the Hardy–Weinberg equilibrium, while linkage disequilibrium was detected between three loci. Cross-species amplification tests suggested that the transferability of ten loci was positive for the two congeners D. japonica and D. ghilarovi. This set of microsatellite markers may be used to evaluate the genetic diversity and population structures of D. gisti and related species in the future.

（Drawida gisti）是土壤的重要生态前哨物种，广泛分布于整个东亚。但是，这种earth的遗传多样性研究所需的分子工具仍然很少。这项研究的目的是开发和表征D. gisti中的微卫星标记，并评估其向其他Drawida物种的转移能力。我们采用RAD-seq方法开发了D. gisti的12个微卫星标记。使用24个D. gisti个体对基因座进行了表征和分析来自自然人口。每个基因座的等位基因数量介于4至11之间，平均为6.5。观察到的杂合度和预期杂合度分别为0.708至0.958和0.568至0.883。没有基因座表现出与Hardy-Weinberg平衡的显着偏离，而在三个基因座之间检测到连锁不平衡。跨物种扩增测试表明，十个基因座的可转移性对于两个同源种（D. japonica）和D. ghilarovi都是阳性的。这套微卫星标记物可用于将来评估D. gisti和相关物种的遗传多样性和种群结构。