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Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-09-14 , DOI: 10.1007/s00604-020-04547-6
Mahmoud Roushani 1 , Zeinab Rahmati 1 , Mehdi Golchin 2 , Zinat Lotfi 2 , Mostafa Nemati 3
Affiliation  

A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of Staphylococcus aureus (S. aureus). Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the S. aureus in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)6]4−/3− is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 107 CFU mL−1 with a detection limit of 3.3 CFU mL−1 with RSD 3.0%. It is capable to accurately determine S. aureus in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect S. aureus based on an electrochemical method. Graphical abstract

中文翻译:

电沉积金纳米粒子固定在玻碳电极上的IgY检测金黄色葡萄球菌的电化学免疫传感器

基于共价连接的抗蛋白 A 抗体 (IgY) 在沉积的金纳米粒子 (AuNP) 修饰的玻璃碳电极 (GCE) 上提出了一种新的超灵敏免疫传感器,用于金黄色葡萄球菌 (S. aureus) 的电化学测量。作为捕获抗体的鸡 IgY 提供与目标细菌的高度选择性和特异性结合,并在其三维空间中选择性地捕获金黄色葡萄球菌。因此可以消除产蛋白G链球菌的干扰。此外,[Fe(CN)6]4-/3- 的电子转移特性受到这种组合的阻碍;因为它反映在电化学阻抗谱 (EIS) 和循环伏安 (CV) 曲线上。所提出的免疫传感器显示出从 10 到 107 CFU mL-1 的宽线性动态范围,检测限为 3。3 CFU mL-1,RSD 3.0%。它能够准确测定作为复杂基质样品的牛奶和人血清中的金黄色葡萄球菌,回收率约为 97-103%。免疫传感器还显示出对其他细菌的高选择性和可接受的稳定性。据推测,我们的研究可以被视为第一个报告鸡 IgY 以基于电化学方法检测金黄色葡萄球菌的研究。图形概要
更新日期:2020-09-14
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