Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest. Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated. We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes. The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Retrospectively registered.

中文翻译：

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ANGPTL4的过表达抑制肿瘤细胞的粘附和迁移，并预测三阴性乳腺癌的良好预后。

三阴性乳腺癌（TNBC）患者的临床结局相对较差。预测TNBC患者预后的标志物可帮助指导治疗。大量证据表明，血管生成素样4（ANGPTL4）参与癌症生长，转移和血管生成的调节。因此，它在TNBC中的作用令人关注。方法：我们通过免疫组织化学（IHC）检测了肿瘤组织中ANGPTL4的表达水平，并检测了其与TNBC患者临床特征的关系。接下来，研究了ANGPTL4对TNBC细胞迁移和粘附的作用和机制。我们发现ANGPTL4过表达与TNBC患者的良好预后相关。ANGPTL4上调在体外抑制细胞粘附，迁移和侵袭。进一步的分析表明，可能的机制可能涉及通过与细胞外基质相关基因相互作用来抑制TNBC进程。目前的发现表明，ANGPTL4表达的增强可能与TNBC的进展呈负相关。ANGPTL4是TNBC的有前途的标志物，应在进一步研究中进行评估。追溯注册。