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A CYC/TB1 type TCP transcription factor controls spikelet meristem identity in barley (Hordeum vulgare L.).
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2020-09-11 , DOI: 10.1093/jxb/eraa416
Yi Shang 1, 2 , Lu Yuan 3 , Zhaocan Di 3 , Yong Jia 4 , Zhenlan Zhang 1 , Sujuan Li 5 , Liping Xing 3 , Zengjun Qi 3 , Xiaoyun Wang 6 , Jinghuan Zhu 2 , Wei Hua 2 , Xiaojian Wu 2 , Minqiu Zhu 3 , Gang Li 7, 8 , Chengdao Li 4, 9
Affiliation  

Barley possesses a branchless, spike-shaped inflorescence where determinate spikelets attach directly to the main axis, but the developmental mechanism of spikelet identity remains largely unknown. Here we report the functional analysis of the barley gene BRANCHED AND INDETERMINATE SPIKELET 1 (BDI1), which encodes a TCP transcription factor and plays a crucial role in determining barley inflorescence architecture and spikelet development. The bdi1 mutant exhibited indeterminate spikelet meristems that continued to grow and differentiate after producing a floret meristem; some spikelet meristems at the base of the spike formed two fully developed seeds or converted to branched spikelets, producing a branched inflorescence. Map-based cloning analysis showed that this mutant has a deletion of ~600 kb on chromosome 5H containing three putative genes. Expression analysis and virus-induced gene silencing confirmed that the causative gene, BDI1, encodes a CYC/TB1-type TCP transcription factor and is highly conserved in both wild and cultivated barley. Transcriptome and regulatory network analysis demonstrated that BDI1 may integrate regulation of gene transcription cell wall modification and known trehalose-6-phosphate homeostasis to control spikelet development. Together, our findings reveal that BDI1 represents a key regulator of inflorescence architecture and meristem determinacy in cereal crop plants.

中文翻译:

CYC / TB1型TCP转录因子控制大麦(Hordeum vulgare L.)中的小穗分生组织身份。

大麦具有无分支,穗状的花序,其中确定的小穗直接附着在主轴上,但小穗同一性的发育机理仍然未知。在这里,我们报告大麦基因分支和不确定小穗1BDI1)的功能分析,其编码TCP转录因子,并在确定大麦花序结构和小穗发育中起关键作用。该bdi1突变体表现出不确定的小穗分生组织,在产生小花分生组织后继续生长并分化。在穗的基部的一些小穗分生组织形成了两个完全发育的种子或转化为分支的小穗,产生了分支的花序。基于图谱的克隆分析表明,该突变体在含有三个推定基因的5H染色体上缺失了〜600 kb。表达分析和病毒诱导的基因沉默证实了致病基因BDI1,编码CYC / TB1型TCP转录因子,在野生和栽培大麦中均高度保守。转录组和调控网络分析表明,BDI1可能整合基因转录细胞壁修饰的调控和已知的6磷酸海藻糖稳态,以控制小穗发育。总之,我们的发现表明BDI1代表着谷类作物植物花序结构和分生组织确定性的关键调节剂。
更新日期:2020-09-11
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