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Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure.
International Archives of Allergy and Immunology ( IF 2.8 ) Pub Date : 2020-09-14 , DOI: 10.1159/000510118
Ruperto González-Pérez 1, 2 , Paloma Poza-Guedes 3, 4 , Fernando Pineda 5 , Peter Forstenlechner 6 , Miriam Castillo 5 , Elena Mederos-Luís 3 , Martina Aumayr 6 , Víctor Matheu 3 , Cristina Alava-Cruz 3 , Inmaculada Sánchez-Machín 3
Affiliation  

Background: The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in the concurrent asthma phenotypes has not been determined. Objective: The aim of the present study lays on a component-resolved diagnosis (CRD) model to investigate the mite molecular signature in subjects with type-2 inflammation asthma. Methods: We selected patients showing a clinically relevant sensitization to HDMs with moderate-to-severe persistent asthma. Skin prick test (SPT) with standardized mite extracts, a broad customized CRD serum sIgE panel including 9 Dermatophagoides pteronyssinus allergens and the related protein allergenic characterization, was investigated in all serum samples. Results: Ninety out of 93 (96.77%) patients with a positive SPT to HDM showed a concordant sIgE (≥0.35 kUA/L) to the crude extract of D. pteronyssinus. Major allergens (Der p 2, Der p 23, and Der p 1) were present in #x3e;70% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 51% in the present cohort. A complex pleomorphic repertoire of HDM molecules recognized by IgE was depicted, including 38 distinct profiles. Conclusions and Clinical Relevance: The proposed CRD panel approach, containing the most prevalent HDM allergens, appeared to be sufficient to obtain a precise D. pteronyssinus molecular diagnosis in asthmatics with a climate-dependent high-mite allergen exposure and coexisting sensitization. A dominant role of both major and mid-tier allergens has been confirmed in moderate and severe persistent asthmatics with the preponderant Th2-high endotype.
Int Arch Allergy Immunol


中文翻译:

暴露于高过敏原的 Th2 极化中度至重度持续性哮喘内型中的螨分子特征。

背景:在并发哮喘表型中,对主要房尘螨 (HDM) 过敏原组的 IgE 反应之间的关联尚未确定。目的:本研究的目的是建立一个组件解析诊断 (CRD) 模型,以研究 2 型炎症性哮喘患者的螨分子特征。方法:我们选择了对中度至重度持续性哮喘表现出对 HDM 具有临床相关敏感性的患者。在所有血清样品中使用标准化螨提取物进行皮肤点刺试验 (SPT),这是一个广泛的定制 CRD 血清 sIgE 面板,包括 9 种屋尘螨过敏原和相关的蛋白质过敏原特征。结果:93 名 (96.77%) 对 HDM 呈阳性 SPT 的患者中的 90 名显示与屋尘螨粗提物一致的 sIgE (≥0.35 kU A / L) 。#x3e 中存在主要过敏原(Der p 2、Der p 23 和 Der p 1);占所有受试者的 70%,中等过敏原(Der p 5、Der p 7 和 Der p 21)高达在本队列中为 51%。描述了由 IgE 识别的 HDM 分子的复杂多形性库,包括 38 个不同的配置文件。结论和临床相关性:提议的 CRD 小组方法,包含最普遍的 HDM 过敏原,似乎足以获得精确的D. pteronyssinus具有气候依赖性高螨过敏原暴露和共存致敏的哮喘患者的分子诊断。主要和中层过敏原在中度和重度持续性哮喘患者中的主导作用已被证实,其中 Th2 高内型占优势。
Int Arch 过敏免疫
更新日期:2020-09-14
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