Abstract

Essential, non-essential and conditionally essential amino acid blood concentrations play a critical role in newborns. We aimed to quantitate most of these amino acids in the blood of full-term breastfed infants, perinatally and correlate the obtained values with their birth weight. Breastfed full-term infants (n = 12,000; 6000 males, 6000 females) with birth weight 2000–4000 g were divided into 4 equal groups: Group A, 2000–2500 g; B, 2500–3000 g; C, 3000–3500 g and D, 3500–4000 g. Blood samples as Dried Blood Spots (DBS) were collected on the 3rd day of life and analyzed via liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol. Blood concentrations of the amino acids, Phenylalanine, Leucine, Glutamine, Ornithine, Alanine, Tyrosine and Glycine in full-term breastfed newborns, were found to be related to their birth weight, perinatally. On the contrary, no relationship between birth weight and blood concentrations of the amino acids Valine, Methionine, Citrulline and Arginine was found. Due to the number of the samples, data from this study could be applied as neonatal screening reference values for full-term breastfed newborns in relation to their birth weight.

摘要

必需、非必需和条件必需氨基酸的血液浓度在新生儿中起着关键作用。我们的目的是在围产期对足月母乳喂养婴儿血液中的大部分氨基酸进行定量，并将获得的值与他们的出生体重相关联。母乳喂养的足月婴儿 ( n = 12,000；6000 名男性，6000 名女性）出生体重 2000-4000 g 被分成 4 个相等的组：A 组，2000-2500 g；B、2500–3000 克；C，3000–3500 克和 D，3500–4000 克。在生命的第 3 天收集作为干血斑 (DBS) 的血样，并通过液相色谱-串联质谱 (LC-MS/MS) 协议进行分析。发现足月母乳喂养的新生儿的苯丙氨酸、亮氨酸、谷氨酰胺、鸟氨酸、丙氨酸、酪氨酸和甘氨酸的血液浓度与其围产期的出生体重有关。相反，未发现出生体重与氨基酸缬氨酸、蛋氨酸、瓜氨酸和精氨酸的血液浓度之间的关系。由于样本数量，