Abstract

One new virginiamycin derivative, ‘beilunmycin’ (1), and three known virginiamycin antibiotics, 16-hydroxy-virginiamycin M1 (2), virginiamycin M2 (3), and virginiamycin M1 (4), were isolated from the culture of a mangrove-derived endophytic Streptomyces sp. 2BBP-J2. The structures were characterized on the basis of their spectroscopic data, and the absolute configuration of 1 was established by ECD calculations. Compounds 1–4 exhibited antibacterial activities against Gram-positive bacteria, with MIC values in the range of 0.5–16 μg/ml. All the compounds demonstrated strong protein translation-stalling activity, with minimal concentrations detected with pDualrep2 in the range of 1.9–5.9 nmol.

摘要

从红树林的培养物中分离出一种新的维吉尼亚霉素衍生物“贝伦霉素”( 1 ) 和三种已知的维吉尼亚霉素抗生素 16-羟基维吉尼亚霉素 M1 ( 2 )、维吉尼亚霉素M2 ( 3 ) 和维吉尼亚霉素M1 ( 4 )。衍生的内生链霉菌属。2BBP-J2。根据光谱数据对结构进行表征，并通过 ECD 计算确定1的绝对构型。化合物1 – 4对革兰氏阳性菌表现出抗菌活性，MIC 值在 0.5-16 μg/ml 范围内。所有化合物都表现出很强的蛋白质翻译停滞活性，pDualrep2 检测到的最低浓度范围为 1.9-5.9 nmol。