BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details. OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment. METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex. FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species. MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank.

中文翻译：

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Nyssomyia属和Lutzomyia亚属的密切相关的phtobotomine沙蝇（Diptera：Psychodidae）的亲缘关系。

背景技术奈瑟菌属和Lutzomyia亚属包括医学上重要的物种，它们是拉丁美洲的利什曼原虫病载体。对于这些分类学组中每一个在形态学上无法区分或通过非常细微的细节区分的密切相关物种的系统发生关系，人们所知甚少。目的我们利用细胞色素C氧化酶亚基I（COI）片段推断夜蛾属和Lutzomyia亚属中密切相关物种的系统发育关系。方法从11个阿根廷地区进行采样。对于遗传分析，除了阿根廷的序列外，我们还使用了GenBank序列。计算Nyssomyia属的密切相关物种之间的Kimura 2参数（K2P）遗传距离和核苷酸差异（Da），Lutzomyia亚属和Lutzomyia longipalpis复合体的进化枝之间。研究结果发现，奈瑟菌属和Lutzomyia亚属种内的K2P和Da值均低于Lu进化枝之间的差异。longipalpis复合体。Lutzomyia亚属内的单倍型网络分析显示物种之间共有单倍型，这与Nyssomyia属相反，没有单倍型共有。Nyssomyia属中的贝叶斯推断按物种进行结构化。主要结论这项研究证明了奈瑟菌属和Lutzomyia亚属内密切相关物种之间的系统发育接近性。来自本研究的奈瑟氏菌的COI序列是GenBank中第一个可用的序列。研究结果发现，奈瑟菌属和Lutzomyia亚属种内的K2P和Da值均低于Lu进化枝之间的差异。longipalpis复合体。Lutzomyia亚属内的单倍型网络分析显示物种之间共有单倍型，这与Nyssomyia属相反，没有单倍型共有。Nyssomyia属中的贝叶斯推断按物种进行结构化。主要结论这项研究证明了奈瑟菌属和Lutzomyia亚属内密切相关物种之间的系统发育接近性。来自本研究的奈瑟氏菌的COI序列是GenBank中第一个可用的序列。研究结果发现，奈瑟菌属和Lutzomyia亚属种内的K2P和Da值均低于Lu进化枝之间的差异。longipalpis复合体。Lutzomyia亚属内的单倍型网络分析显示物种之间共有单倍型，这与Nyssomyia属相反，没有单倍型共有。Nyssomyia属中的贝叶斯推断按物种进行结构化。主要结论这项研究证明了奈瑟菌属和Lutzomyia亚属内密切相关物种之间的系统发育接近性。来自本研究的奈瑟氏菌的COI序列是GenBank中第一个可用的序列。与Nyssomyia属相反，没有单倍型共享。Nyssomyia属中的贝叶斯推断按物种进行结构化。主要结论这项研究证明了奈瑟菌属和Lutzomyia亚属内密切相关物种之间的系统发育接近性。来自本研究的奈瑟氏菌的COI序列是GenBank中第一个可用的序列。与Nyssomyia属相反，没有单倍型共享。Nyssomyia属中的贝叶斯推断按物种进行结构化。主要结论这项研究证明了奈瑟菌属和Lutzomyia亚属内密切相关物种之间的系统发育接近性。来自本研究的奈瑟氏菌的COI序列是GenBank中第一个可用的序列。