The circadian clock regulates vital aspects of physiology including protein synthesis and oxidative stress response. In this investigation, we performed a proteome‐wide scrutiny of rhythmic protein accrual in Drosophila melanogaster on exposure to rotenone, rotenone + hesperidin and hesperidin in D. melanogaster. Total protein from fly samples collected at 6 h intervals over the 24 h period was subjected to two‐dimensional gel electrophoresis and mass spectrometry. Bioinformatics tool, Protein ANalysis THrough Evolutionary Relationships classification system was used to the determine the biological processes of the proteins of altered abundance. Conspicuous variations in the proteome (151 proteins) of the flies exposed to oxidative stress (by rotenone treatment) and after alleviating oxidative stress (by hesperidin treatment) were observed during the 24 h cycle. Significantly altered levels of abundance of a wide variety of proteins under oxidative stress (rotenone treatment) and under alleviation of oxidative stress (rotenone + hesperidin treatment) and hesperidin (alone) treatment were observed. These proteins are involved in metabolism, muscle activity, heat shock response, redox homeostasis, protein synthesis/folding/degradation, development, ion‐channel/cellular transport, and gustatory and olfactory function of the flies. Our data indicates that numerous cellular processes are involved in the temporal regulation of proteins and widespread modulations happen under rotenone treatment and, action of hesperidin could also be seen on these categories of proteins.

中文翻译：

---

橙皮苷调节果蝇在氧化应激下的节律性蛋白质组谱。

昼夜节律调节生理的重要方面，包括蛋白质合成和氧化应激反应。在这项研究中，我们对果蝇中的鱼藤酮，鱼藤酮+橙皮苷和橙皮苷进行了蛋白质组学检查，对果蝇的节律性蛋白进行了详细的研究。。在24小时内以6小时间隔采集的蝇样品中的总蛋白经过二维凝胶电泳和质谱分析。使用生物信息学工具“蛋白质分析通过进化关系分类”系统确定丰度变化的蛋白质的生物学过程。在24小时的周期中，观察到暴露于氧化应激（通过鱼藤酮处理）和减轻氧化应激（通过橙皮苷处理）的果蝇的蛋白质组（151种蛋白质）存在明显变化。观察到在氧化应激（鱼藤酮处理）和缓解氧化应激（鱼藤酮+橙皮苷处理）和橙皮苷（单独）处理的情况下，各种蛋白质的丰度水平发生了显着变化。这些蛋白质参与新陈代谢，肌肉活动，热休克反应，氧化还原稳态，蛋白质合成/折叠/降解，发育，离子通道/细胞运输以及果蝇的味觉和嗅觉功能。我们的数据表明，蛋白质的时间调控涉及许多细胞过程，并且在鱼藤酮处理下会发生广泛的调节作用，橙皮苷的作用也可以在这些类别的蛋白质上看到。