当前位置: X-MOL 学术J. Fish Dis. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A C-type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus.
Journal of Fish Diseases ( IF 2.5 ) Pub Date : 2020-09-13 , DOI: 10.1111/jfd.13255
Xiao-Wen Zhang 1 , Cong-Hui Yang 1 , Hong-Quan Zhang 2 , Xin-Tong Pan 1 , Ze-Yu Jin 1 , Hong-Wei Zhang 3 , Xiao-Hua Xia 1
Affiliation  

C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific QPD motif and a conserved Ca2+‐binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+‐dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach.

中文翻译:

一种C型凝集素,在天气泥ach Misgurnus anguillicaudatus中具有抗菌活性。

C型凝集素是碳水化合物结合蛋白,通过充当模式识别受体而在免疫中发挥重要作用。在本研究中,从天气泥ach Misgurnus anguillicaudatus(称为MaCTL )中获得了一种新型的纳豆素样C型凝集素。MaCTL编码具有165个氨基酸的肽,具有信号肽和单个C型凝集素结构域(CTLD),其中包含半乳糖特异性QPD基序和保守的Ca 2+结合位点。MaCTL的转录产物在对其病原体亲水链球菌免疫攻击后明显上调。用重组MaCTL蛋白进行的体外测定表明,它在Ca中具有血凝和细菌凝集活性。2+依赖方式。发现MaCTL与多种细菌结合,并与细菌多糖LPS和PGN结合。此外,MaCTL通过抑制细菌的生长显示出抗菌活性。这些结果共同表明,MaCTL参与了天气泥ach的抗菌防御。
更新日期:2020-11-06
down
wechat
bug