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Evaluation of an efficient fed-batch enzymatic hydrolysis strategy to improve production of functional xylooligosaccharides from maize straws
Industrial Crops and Products ( IF 5.9 ) Pub Date : 2020-09-13 , DOI: 10.1016/j.indcrop.2020.112920
Shiquan Qian , Jing Zhou , Xiangyu Chen , Wei Ji , Luyao Zhang , Weicheng Hu , Zhaoxin Lu

The present study investigated the production of xylooligosaccharides (XOS) from the xylans extracted from maize straws (XMSs) using the fed-batch enzymatic hydrolysis strategy (FBEH). When fed 300 mL of the fresh XMSs with 2 % of the substrate loading and 12 U/g of the enzyme activity at the enzymatic hydrolysis time of 7 h, the yield of XOS was obtained to be 0.67 g/g of XMSs. Characterization analysis indicated that XOS contained typical molecular groups and side chains of oligosaccharides and was mainly composed of xylobiose, xylotriose and xylose. Furthermore, XOS exhibited strong antioxidant capacity in vitro and had a significant inhibition effect on HepG2 cells, indicating that XOS might be potentially used as natural antioxidants and anticancer additives in food or pharmaceutical industry.



中文翻译:

评估提高玉米秸秆功能木糖寡糖生产效率的分批补料酶解策略

本研究使用补料分批酶解策略(FBEH)研究了从玉米秸秆(XMSs)中提取的木聚糖产生的木寡糖(XOS)。当在7 h的酶水解时间下进料300 mL具有2%底物负载和12 U / g酶活性的新鲜XMS时,XOS的产量为0.67 g / g XMS。表征分析表明,XOS含有典型的寡糖分子和侧链,主要由木糖,木三糖和木糖组成。此外,XOS在体外具有很强的抗氧化能力并且对HepG2细胞具有明显的抑制作用,这表明XOS可能在食品或制药工业中用作天然抗氧化剂和抗癌添加剂。

更新日期:2020-09-14
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