Tobacco is an excellent model plant to study developmental processes and cellular biology. Surface dielectric barrier discharge (sDBD) was used to generate plasma activated water (PAW), and the PAW was indirectly applied to study tobacco plant growth during the early developmental stages. Solid media were treated with the PAW for 8- and 18-day-old seedlings, and the effects on both the vegetative and root phenotypes were observed during the early developmental stages. In the vegetative tissue, the cotyledon size was large for the seedling that was cultivated by plasma activated water for the 12 min PAW (PAW12) treatment. The PAW dramatically elongated the cotyledon by 40% due to increased cell expansion in the leaf-length direction, not cell proliferation. In addition to the vegetative tissue, the root length due to the PAW was significantly enhanced at the primary root with elongated root hairs. According to molecular analysis, the NT_ COBRA-LIKE 9 (COBL) and two NT_ XYLOGLUCAN ENDO-TRANSGLYCOSYLASE/HYDROLASE (XTH) genes, NT_XTH9 and NT_XTH15, were significantly over-expressed by the PAW treatment compared with untreated deionized water (DW), indicating that the PAW effects are from modulating the expression of target genes. These results demonstrate that PAW might promote tobacco plant growth both in the cotyledon and root hair tissues via the NT_COBL, NT_XTH9 and NT_XTH15 genes.

烟草是研究发育过程和细胞生物学的优秀典范植物。表面介电势垒放电（sDBD）用于产生等离子体活化水（PAW），并且该PAW间接用于研究烟草植物在早期发育阶段的生长。用PAW处理固体培养基，分别处理8天和18天的幼苗，并在发育的早期阶段观察到对营养和根表型的影响。在营养组织中，子叶的大小对于用等离子活化水进行12分钟PAW（PAW12）处理的幼苗来说是很大的。PAW显着延长了子叶40％，这是由于沿叶长方向的细胞扩增增加，而不是细胞增殖。除了营养组织 由于PAW引起的根长在原发根处显着增加，且根毛拉长。根据分子分析，与未处理的去离子水（DW）相比，PAW处理显着过量表达了NT_ COBRA-LIKE 9（COBL）和两个NT_ XYLOGLUCAN内切-糖基转移酶/水合酶（XTH）基因NT_XTH9和NT_XTH15。调节靶基因的表达。这些结果表明，PAW可能通过NT_COBL，NT_XTH9和NT_XTH15基因在子叶和根毛组织中促进烟草植物的生长。