当前位置: X-MOL 学术J. Nanopart. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Cell biophysical characteristics of PPy-GNPs and their application in photothermal therapy of SKOV-3 cell
Journal of Nanoparticle Research ( IF 2.5 ) Pub Date : 2020-09-14 , DOI: 10.1007/s11051-020-04993-6
Chuanzhi Liu , Yuxi Huang , Chunru Zhao , Fan Yang , Haiqi Qin , Zuobin Wang

The pursuit of multifunctional, stable, more efficient, and safer cancer treatment has gained increasing interest in the research of nanoparticle-mediated photothermal therapy. Many nanoparticles have good absorption chracteristics in the near-infrared region, because of the high light-to-heat conversion efficiency; they could destroy tumor cells by heat; thus, they are often treated as a potential photothermal therapy agent. In this work, a kind of polypyrrole (PPy)-coated gold nanoparticles particles (PPy-GNPs) with high photothermal conversion capability was prepared. Their particle sizes were about 90 ± 11.2 nm. The temperature of PPy-GNPs solution in a concentration of 0.25 mg/ml rises 13.6 °C, and the nanoparticles could significantly inhibit the proliferation of SKOV-3 cells under irradiation of 25 W·cm−2 laser (808 nm) for 10 min, with the maximum cell viability value is maintained at 87.6% by MTT assay. In the same photothermal and cultured conditions, the photothermal conversion efficiency and cytostatic effect of the gold nanoparticle are much lower than that of the PPy-GNPs group. The atomic force microscopy (AFM) was used to identify the function of nanoparticles for the structural changes of the cells. The SKOV-3 cell of adhesion forces decrease by 21%, and Young’s modulus increases by 26% compared with control. These results suggest that PPy-GNPs are effective targeted photothermal therapeutic agents for cancer treatment.



中文翻译:

PPy-GNP的细胞生物物理特性及其在SKOV-3细胞光热疗法中的应用

对多功能,稳定,更有效和更安全的癌症治疗方法的追求已引起人们对纳米粒子介导的光热疗法研究的兴趣。由于高的光热转换效率,许多纳米粒子在近红外区域具有良好的吸收特性。它们可以通过加热破坏肿瘤细胞;因此,它们经常被当作潜在的光热疗法治疗剂。本工作制备了一种具有高光热转化能力的聚吡咯(PPy)包覆的金纳米颗粒(PPy-GNPs)。它们的粒径为约90±11.2nm。浓度为0.25 mg / ml的PPy-GNPs溶液的温度升高13.6°C,并且纳米粒子可以在25 W·cm -2的辐射下显着抑制SKOV-3细胞的增殖。激光(808 nm)照射10分钟,通过MTT分析将最大细胞活力值保持在87.6%。在相同的光热和培养条件下,金纳米颗粒的光热转化效率和细胞抑制作用远低于PPy-GNPs组。原子力显微镜(AFM)用于鉴定纳米颗粒对细胞结构变化的作用。与对照相比,SKOV-3细胞的粘附力降低了21%,杨氏模量提高了26%。这些结果表明,PPy-GNP是用于癌症治疗的有效靶向光热治疗剂。

更新日期:2020-09-14
down
wechat
bug