Although nickel (Ni) has direct relationship with nitrogen metabolism of plants, the high dose of Ni fertilizer in broad bean plants may affect the nitrogen use efficiency (NUE), impair plant development and even cause Ni pollution in soil. Thus, a pot experiment was set up to study the effect of urea fertilization on N-uptake, root and shoots’ Ni content as well as growth of broad bean plants under different levels of Ni, using ¹⁵N tracer technique. ¹⁵N-labeled urea (5% ¹⁵N atom excess) was added at three doses (0, 30 and 60 mg N kg⁻¹ soil). Nickel sulfate (NiSO₄) was also applied at three levels (0, 50 and 100 mg Ni kg⁻¹ soil). The experiment was laid out with or without acetic acid in randomized complete block design in three replicates. Treatment with the addition of 60 mg N + 50 mg Ni showed the highest values in dry weights of root and shoots, N-uptake by shoots, nitrogen derived from fertilizer (Ndff %) and NUE % by shoots in both with or without acetic acid solution. Higher rate of Ni addition can decrease shoot and root biomass by inhibiting the ability of the plant to uptake the nitrogen efficiently. However, addition of acetic acid solution induced the improvement of NUE % and Ndff % by shoot and root of broad bean plants. This study provides insight into how to improve plant yield without damaging the soil health and will be helpful to create a better world with sustainable agriculture.

尽管镍（Ni）与植物的氮代谢有直接关系，但是蚕豆植物中高剂量的镍肥可能会影响氮的利用效率（NUE），损害植物的生长，甚至造成土壤中的Ni污染。因此，建立了盆栽试验，使用¹⁵ N示踪技术研究了尿素施肥对氮素吸收，根和芽的Ni含量以及蚕豆在不同Ni水平下的生长的影响。以三种剂量（0、30和60 mg N kg ^-1土壤）添加¹⁵ N标记的尿素（过量¹⁵ N原子5％）。还以三种水平（0、50和100 mg Ni kg ^-1）施用硫酸镍（NiSO ₄）泥）。实验在有或没有乙酸的情况下进行，以随机重复的完整模块设计进行，一式三份。在添加或不添加乙酸的情况下，添加60 mg N + 50 mg Ni处理后，根和枝条的干重，枝条的氮吸收量，肥料中的氮素含量（Ndff％）和枝条的NUE％最高。解。较高的镍添加速率可以通过抑制植物有效吸收氮的能力来减少枝条和根系生物量。然而，添加乙酸溶液诱导蚕豆植株的根和茎的NUE％和Ndff％提高。这项研究提供了有关如何在不损害土壤健康的情况下提高植物产量的见识，并将有助于通过可持续农业创造更美好的世界。