The effects of electromagnetic fields on plants have been studied during the last decades. The electromagnetic fields as abiotic stress can induce genetic variation in living organisms including plants by different mechanisms. The aim of the present study is to assay the possible electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. which is known as favorite herbal beverages by many biochemical constituents. To do so, intersimple sequence repeats (ISSR) and start codon targeted (SCoT) loci were used as molecular markers. Different electromagnetic field treatments by 1, 2, 4 and 6 mT of intensity, for 30 and 60 min per day, for 7 continuous days were imposed, following by DNA extraction and PCR amplification. AMOVA, PCoA and STRUCTURE analysis based on the ISSR and SCoT markers showed that regardless of the EMFs intensity, 30 min of radiation causes more genetic variation than that of 60 min does until the intensity was increased up to 6 mT, due to direct and indirect mechanisms such as guanine modification, methylation of cytosines, Fenton reaction and direct DNA strands breaking. ISSR sequences, however, underwent more variation than SCoT.

中文翻译：

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利用ISSR和SCoT标记评估电磁场引起的茶茶树遗传多样性

在过去的几十年里，人们一直在研究电磁场对植物的影响。作为非生物胁迫的电磁场可以通过不同的机制诱导包括植物在内的生物体的遗传变异。本研究的目的是测定可能由电磁场引起的 Tea Camellia sinensis L. 的遗传多样性，它被许多生化成分称为最受欢迎的草药饮料。为此，使用简单序列重复 (ISSR) 和起始密码子靶向 (SCoT) 基因座作为分子标记。施加强度为 1、2、4 和 6 mT 的不同电磁场处理，每天 30 和 60 分钟，连续 7 天，然后进行 DNA 提取和 PCR 扩增。基于 ISSR 和 SCoT 标记的 AMOVA、PCoA 和 STRUCTURE 分析表明，无论 EMF 强度如何，由于直接和间接机制，如鸟嘌呤修饰、胞嘧啶甲基化、芬顿反应和直接 DNA 链断裂，30 分钟的辐射比 60 分钟的辐射导致更多的遗传变异，直到强度增加到 6 mT。然而，ISSR 序列比 SCoT 经历了更多的变化。