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Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil
Brazilian Journal of Botany ( IF 1.6 ) Pub Date : 2020-09-14 , DOI: 10.1007/s40415-020-00647-y
Vitor Cunha Manhães , Dayvid Rodrigues Couto , Fabiano Salgueiro , Andrea Ferreira da Costa

Stigmatodon Leme, G.K.Br. & Barfuss is one of the most representative Bromeliaceae genera that grow on the steepest slopes of the inselbergs of southeastern Brazil. Inselberg environments are characterized as islands due to their restricted, disjunct distribution and geographical isolation. These features make these sites interesting models for evolutionary and population genetic studies. In this study we report, the cross-species transferability of nuclear microsatellite markers, originally developed for other Bromeliaceae, to four Stigmatodon species: Stigmatodon brassicoides, S. costae, S. goniorachis and Stigmatodon sp. nov. Twenty-four microsatellite markers were tested, and 12 of these were successfully amplified in the four studied species. In S. brassicoides and S. costae, 10 markers showed amplification with polymorphism, and in Stigmatodon sp. nov and S. goniorachis, all 12 markers showed polymorphisms. The number of alleles ranged from two to four in S. brassicoides; from two to three in S. costae; from two to six in Stigmatodon sp. nov; and from two to seven in S. goniorachis. HO values ranged from 0.05 (P2P19 locus) in S. brassicoides to 0.689 (VgA04 locus) in S. goniorachis. The set of amplified microsatellite markers described in this study will be a useful tool for addressing various evolutionary, ecological and conservation aspects of Stigmatodon species.



中文翻译:

核微卫星标记对柱头兽物种的可转移性:巴西的Inselbergs垂直悬崖特有的凤梨属

柱头目莱姆(Stigmatodon Leme),GKBr。&Barfuss是生长在巴西东南部inselbergs最陡峭山坡上的最具代表性的凤梨科之一。因赛尔贝格(Inselberg)环境的局限性,分散的分布和地理上的隔离,其特点是岛屿。这些特征使这些位点成为进化和种群遗传研究的有趣模型。在这项研究中,我们报告,核微卫星标记的跨物种转让,原本为其他凤梨开发,四个Stigmatodon种:Stigmatodon brassicoidesS.肋脉S. goniorachisStigmatodonsp。十一月 测试了二十四个微卫星标记,并在四个研究物种中成功扩增了其中的十二个。在S. brasicoidesS. costae中,10个标记显示出具有多态性的扩增,在Stigmatodon sp。nov和S. goniorachis,所有12个标记均显示多态性。芸苔S.等位基因的数量范围为2至4个S. costae中从2到3 ; 从Stigmatodon sp。的2到6 。十一月 在S. goniorachis中从2到7 。H O值的范围从S. brasicoides中的0.05(P2P19基因座)到0.689(VgA04基因座)中。S. goniorachis。该组在这项研究中进行扩增微卫星标记的将是定址的各种进化,生态和保育方面的有用工具Stigmatodon种。

更新日期:2020-09-14
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