We hereby present a concept of scavenging excess imaging agent prior to a diagnostic imaging session, consequently allowing for enhanced contrast of signals originating from the tissue area of interest to the signals originating from systemic imaging agent residues. In our study, a prospective silica core–shell nanoparticle-based scavenger was designed and explored for its feasibility to scavenge a specific imaging agent (tracer) in the bloodstream. The developed tracer–scavenger system was first investigated under in vitro conditions to ensure proper binding between tracer and scavenger is taking place, as confirmed by Förster/fluorescence resonance energy transfer studies. In vivo, two-photon imaging was utilized to directly study the interaction of the scavenger particles and the tracer molecules in the vasculature of mice. To our knowledge, a methodological solution for in vivo differentiation between signals, originating from tissue and blood, has not been presented elsewhere.

我们在此提出在诊断成像会议之前清除过量成像剂的概念，从而使源自感兴趣组织区域的信号与源自全身成像剂残留物的信号的对比度得到增强。在我们的研究中，设计并研究了一种基于二氧化硅核-壳纳米粒子的前瞻性清除剂，其清除血液中特定成像剂（示踪剂）的可行性。Förster/荧光共振能量转移研究证实，首先在体外条件下研究了开发的示踪剂-清除剂系统，以确保示踪剂和清除剂之间发生适当的结合。体内，利用双光子成像技术直接研究了小鼠脉管系统中清除剂颗粒与示踪剂分子之间的相互作用。据我们所知，尚未在其他地方提出用于体内区分源自组织和血液的信号的方法学解决方案。