Abstract The relationship between protein adsorption on hydroxyapatite (HAp) particles and their surface structure was investigated. Because the various crystal planes of HAp have been reported to exhibit selective adsorption, numerous studies have focused on developing methods to control HAp morphology for selective adsorption. However, few studies have examined the systematic adsorption of proteins on the HAp particles. We firstly synthesized HAp particles under various aging times and mild reaction conditions intending to obtain HAp particles having various surface structures despite similar morphology, chemical composition, and crystallinity. The aging time affected the pore size distribution of the HAp particles. A peak indicating pores with a diameter of approximately 2.5 nm was observed in the pore size distribution plots of the HAp particles prepared using aging times of 48 h or less. The adsorption of proteins on HAp particles with different surface structures was studied. The bovine serum albumin (BSA) adsorption behavior was influenced by the presence of pores on the HAp surface. The amount of BSA adsorbed on the HAp particles aged 72 h having no pores was nearly 1.5 times that of the other HAp particles having pores. These results indicated that the pore size distribution of HAp particles is one of the most important factors in controlling their protein adsorption behavior.

中文翻译：

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羟基磷灰石颗粒孔隙分布对其蛋白质吸附行为的影响

摘要 研究了羟基磷灰石（HAp）颗粒上的蛋白质吸附与其表面结构之间的关系。由于已报道 HAp 的各种晶面表现出选择性吸附，因此许多研究都集中在开发控制 HAp 形态以进行选择性吸附的方法。然而，很少有研究检查蛋白质在 HAp 颗粒上的系统吸附。我们首先在不同的老化时间和温和的反应条件下合成了 HAp 颗粒，旨在获得具有不同表面结构的 HAp 颗粒，尽管它们具有相似的形态、化学组成和结晶度。老化时间影响 HAp 颗粒的孔径分布。表示孔径约为 2 的孔的峰。在使用 48 小时或更短的老化时间制备的 HAp 颗粒的孔径分布图中观察到 5 nm。研究了蛋白质在不同表面结构的 HAp 颗粒上的吸附。牛血清白蛋白 (BSA) 的吸附行为受 HAp 表面孔隙的影响。老化72 h 的无孔HAp 颗粒上的BSA 吸附量是其他有孔HAp 颗粒的近1.5 倍。这些结果表明，HAp 颗粒的孔径分布是控制其蛋白质吸附行为的最重要因素之一。牛血清白蛋白 (BSA) 的吸附行为受 HAp 表面孔隙的影响。老化72 h 的无孔HAp 颗粒上的BSA 吸附量是其他有孔HAp 颗粒的近1.5 倍。这些结果表明，HAp 颗粒的孔径分布是控制其蛋白质吸附行为的最重要因素之一。牛血清白蛋白 (BSA) 的吸附行为受 HAp 表面孔隙的影响。老化72 h 的无孔HAp 颗粒上的BSA 吸附量是其他有孔HAp 颗粒的近1.5 倍。这些结果表明，HAp 颗粒的孔径分布是控制其蛋白质吸附行为的最重要因素之一。