The proposed research study was undertaken to establish a simple, rapid and sensitive high-performance thin-layer chromatography (HPTLC) method for the quantification of trigonelline (TGN) in methanolic, aqueous and hydroalcoholic extracts of different brands of coffee samples with different colours due to roasting time. The proposed HPTLC methodology was carried out using glass coated silica gel 60 F₂₅₄ plates. The mobile phase used was quaternary mixture of ethyl acetate:methanol:water:formic acid 5.4:2.3:1.5:0.8 (%, v/v) which furnished a compact spot of TGN at Rf 0.24 ± 0.01. The developed plate was scanned and quantified densitometrically at 267 nm. Linear regression analysis presented a good relationship between peak area and amount of TGN in the range of 100–700 ng/band. The method was validated as per the International Conference on Harmonization (ICH) recommendations. The maximum amount of TGN was recorded in roasted Arabic coffee among different brands studied. The amounts of TGN in methanolic, aqueous and hydroalcoholic extract of roasted Arabic coffee were obtained as 0.74, 0.80 and 0.79% w/w, respectively. The proposed analytical methodology will be useful to enumerate the therapeutic dose of TGN in different herbal products.

中文翻译：

---

高效薄层色谱法测定沙特阿拉伯市场上各种商用咖啡的各种提取物和不同品种中的三角茶碱含量

进行了拟定的研究，以建立一种简单，快速，灵敏的高效薄层色谱法（HPTLC），用于定量分析不同颜色的不同品牌咖啡样品的甲醇，水和水醇提取物中的三角果碱（TGN）。烤时间。所提出的HPTLC方法学是使用玻璃涂层硅胶60 F ₂₅₄板进行的。所使用的流动相是乙酸乙酯：甲醇：水：甲酸5.4：2.3：1.5：0.8（％，v / v）的季铵盐混合物，在Rf 0处提供了TGN的致密斑点。24  ±  0 。01.扫描显影的板并在267nm处光密度定量。线性回归分析表明，在100–700 ng / band范围内，峰面积与TGN量之间存在良好的关系。该方法已根据国际协调会议（ICH）的建议进行了验证。在研究的不同品牌中，阿拉伯咖啡的烘焙物中记录到的TGN最高。烘焙阿拉伯咖啡的甲醇提取物，水提取物和水醇提取物中的TGN量分别为0.74、0.80和0.79％w / w。所提出的分析方法将有助于列举不同草药产品中TGN的治疗剂量。