Abstract The utilization of waste has gained momentum in the field of waste management and industrial bioprocess. In this study, waste chicken feet were used as the source for extraction of a natural inducer, i.e. gelatin for the synthesis of proteolytic enzyme gelatinase. Microorganisms with gelatinolytic activity were screened from mixed culture isolates obtained from a local poultry waste dumping site. The strain which had shown maximum activity was identified as Brevundimonas vesicularis MF276770 using 16S rRNA gene sequencing. The composition of chicken feet gelatin was analysed and further characterized by Fourier transform infrared analysis and SDS-PAGE. The maximum gelatinase activity of 18.8 U/mL was achieved for the isolated Brevundimonas vesicularis MF276770 at 12 h under optimized conditions of pH 7, substrate concentration (2%), inoculums age (12 h), inoculum volume (2%, v/v), temperature (50 °C) and RPM (140). The enzyme kinetics parameters Vmax and Km were observed as 7.4 U/mL and 0.422 µmol, respectively. The molecular weight of the produced gelatinase was determined as 67 kDa. The produced gelatinase was employed to strip the gelatin silver layer from X-ray polyester film with 1.93 U/mL of gelatinase activity.

中文翻译：

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Brevundimonas vesicularis MF276770，一种利用鸡爪明胶生产明胶酶的新菌株

摘要 废物利用在废物管理和工业生物处理领域获得了发展势头。在这项研究中，废鸡爪被用作提取天然诱导剂的来源，即用于合成蛋白水解酶明胶酶的明胶。从当地家禽废物倾倒场获得的混合培养分离物中筛选出具有明胶溶解活性的微生物。使用 16S rRNA 基因测序将显示出最大活性的菌株鉴定为 Brevundimonas vesicularis MF276770。通过傅里叶变换红外分析和SDS-PAGE分析和进一步表征鸡爪明胶的组成。在 pH 7、底物浓度 (2%)、底物浓度 (2%) 的优化条件下，分离的 Brevundimonas vesicularis MF276770 在 12 小时时达到了 18.8 U/mL 的最大明胶酶活性。接种物年龄 (12 小时)、接种物体积 (2%, v/v)、温度 (50 °C) 和 RPM (140)。观察到的酶动力学参数 Vmax 和 Km 分别为 7.4 U/mL 和 0.422 µmol。所产生的明胶酶的分子量测定为67kDa。使用制备的明胶酶从X射线聚酯薄膜上剥离明胶银层，明胶酶活性为1.93 U/mL。