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Metabolic perturbation of acrylate pathway in Lactobacillus plantarum
Biocatalysis and Biotransformation ( IF 1.8 ) Pub Date : 2019-05-10 , DOI: 10.1080/10242422.2019.1606215
Sowmiya Balasubramanian 1 , Ramalingam Subramanian 1
Affiliation  

Abstract Engineering microbes with heterologous pathway for production of bio-based products has received considerable attention. Reconstituting such non-native pathway in addition to desired product formation often brings an allosteric modulation in enzymes competing at fragile nodes that result in by-product redistribution, in order to retain energy and redox balance. This work, Lactobacillus plantarum engineered with acrylate pathway for propionate production was studied under similar perspectives. Upon expression, the heterologous pathway did not result in propionic acid production under standard glucose concentration of 20 g/L, but 0.01 mM of propionate was formed when grown under low glucose concentration of 1 g/L. Further analysis of secreted metabolites with increased glucose concentration of 10 and 30 g/L remained futile towards propionate formation but showed reorientation in pyruvate metabolism which was related to the control imposed by the host to regulate the hidden constraints caused by gene perturbation. Further, it was ensured that the limitation of supplements did not play any functional role in inhibiting propionic acid synthesis but still followed similar metabolic pattern which was quite unclear though interpreted to certain extent. Thus, the findings gave insights into physiological and metabolic capabilities of Lactobacillus plantarum that at least in principle can be used to enhance the strain performance for increased propionic acid production.

中文翻译:

植物乳杆菌中丙烯酸酯途径的代谢扰动

摘要 具有异源途径的工程微生物用于生产生物基产品受到了相当多的关注。除了所需的产物形成之外,重建这种非天然途径通常会导致在脆弱节点竞争的酶发生变构调节,从而导致副产物重新分配,以保持能量和氧化还原平衡。这项工作是在类似的观点下研究了通过丙烯酸酯途径生产丙酸的植物乳杆菌。表达后,异源途径在 20 g/L 的标准葡萄糖浓度下不会产生丙酸,但在 1 g/L 的低葡萄糖浓度下生长时会形成 0.01 mM 丙酸。对葡萄糖浓度增加 10 和 30 g/L 的分泌代谢物的进一步分析对丙酸的形成仍然无效,但显示丙酮酸代谢的重新定向,这与宿主施加的控制有关,以调节由基因扰动引起的隐藏约束。此外,确保补充剂的限制在抑制丙酸合成方面没有发挥任何功能作用,但仍遵循类似的代谢模式,尽管在一定程度上解释了这一点,但仍不清楚。因此,这些发现让我们深入了解了植物乳杆菌的生理和代谢能力,至少原则上可用于提高菌株性能以增加丙酸产量。
更新日期:2019-05-10
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