Abstract Enzymatic production of L-α-glycerylphosphorylcholine (L-α-GPC) is difficult due to the limited solubility of phosphatidylcholine (PC) in the aqueous phase. Surfactants can be used to improve the solubility and the dispersibility of non-polar chemicals in the aqueous media. In this study, various surfactants were investigated to improve L-α-GPC enzymatic production using phospholipase A1 (PLA1) in the aqueous phase. The results showed that Tween 20 was the most effective surfactant for enhancing L-α-GPC concentration. With 20 g.L−1 of Tween 20, the optimal conditions of PC hydrolysis were determined to be enzyme loading of 0.64 g.L−1 and substrate concentration of 60 g.L−1 at 45 °C for 1 h. In addition, the fed-batch catalytic process of PC was conducted to avoid substrate inhibition and increase product accumulation, resulting in 112.56 g.L−1 of L-α-GPC from 360.00 g.L−1 PC with yield of 91.36% within 3 h.

中文翻译：

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表面活性剂在水相中使用磷脂酶 A1 增强了从磷脂酰胆碱生产 l-α-甘油基磷酸胆碱

摘要 由于磷脂酰胆碱 (PC) 在水相中的溶解度有限，L-α-甘油基磷酸胆碱 (L-α-GPC) 的酶促生产很困难。表面活性剂可用于改善非极性化学品在水性介质中的溶解性和分散性。在这项研究中，研究了各种表面活性剂，以在水相中使用磷脂酶 A1 (PLA1) 来改善 L-α-GPC 酶促生产。结果表明，吐温 20 是提高 L-α-GPC 浓度最有效的表面活性剂。对于 20 gL-1 的吐温 20，PC 水解的最佳条件被确定为 0.64 gL-1 的酶负载和 60 gL-1 的底物浓度，45 °C 下 1 小时。此外，为了避免底物抑制和增加产物积累，进行了 PC 的补料分批催化过程，得到了 112。