Abstract The recent application studies on keratinase lead to new application areas for the enzyme to be used in novel industries such as pharmaceuticals and biodegradable composites. Thus, designing an economical and environmentally friendly keratinase production and purification with new features turned into an intriguing objective for its commercialization. In this study, keratinase enzyme was produced using a bioreactor of 5 L by a native Streptomyces sp. 2M21 and purified by ion exchange chromatography using step-wise gradient with 24.1-fold purification. Purified keratinase showed optimum activity at pH 9 and 30 °C. The enzyme was shown to be able to decompose different complex substrates such as chicken feather, wool and nail. Moreover, the addition of DTT enhanced the hydrolysis of wool and chicken feather to 3.4- and 3.2-fold, respectively. The potential of Streptomyces sp. to produce significant levels of keratinase using feathers as a substrate might establish cleaner applications for the production of high value-added products and degrading of keratin-containing wastes.

中文翻译：

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来自 Streptomyces sp. 的蛋白水解酶的生产、纯化和表征。2M21

摘要 最近对角蛋白酶的应用研究为该酶在制药和可生物降解复合材料等新兴行业中的应用开辟了新的领域。因此，设计具有新功能的经济且环保的角蛋白酶生产和纯化成为其商业化的一个有趣目标。在这项研究中，角蛋白酶是由天然链霉菌属物种使用 5 L 生物反应器生产的。2M21 并通过离子交换色谱法使用逐步梯度进行纯化，纯化率为 24.1 倍。纯化的角蛋白酶在 pH 9 和 30 °C 下显示出最佳活性。该酶被证明能够分解不同的复杂底物，如鸡毛、羊毛和指甲。此外，添加 DTT 将羊毛和鸡毛的水解提高到 3.4 倍和 3.2 倍，分别。链霉菌属的潜力。使用羽毛作为底物生产大量角蛋白酶可能会为高附加值产品的生产和含角蛋白废物的降解建立更清洁的应用。