Abstract Incubation of testosterone 1 with Cladosporium sphaerospermum MRC 70266 afforded six metabolites and two of these metabolites, 6β,16β,17β-trihydroxyandrost-4-en-3-one 6 and 6β,12β,17β-trihydroxyandrost-4-en-3-one 7, were determined as new compounds. The fungus mainly hydroxylated testosterone 1 at C-6β, accompanied by some minor hydroxylations at C-7β, C-12β, C-15α and C-16β. A minor oxidation at C-17 and a minor 5α-reduction were also observed.

中文翻译：

---

Cladosporium sphaerospermum对睾酮的生物转化

摘要 睾酮 1 与枝孢菌 MRC 70266 一起孵育可提供六种代谢物，其中两种代谢物 6β,16β,17β-trihydroxyandrost-4-en-3-one 6 和 6β,12β,17β-trihydroxyandrost-4-en-3 1 7，被确定为新化合物。该真菌主要在 C-6β 处羟基化睾酮 1，伴随着在 C-7β、C-12β、C-15α 和 C-16β 处的一些次要羟基化。还观察到 C-17 处的少量氧化和少量 5α 还原。