As an extremely virulent pathogen, white spot syndrome virus (WSSV) greatly threatens shrimp aquaculture worldwide. The interaction between virus and host is important for viral infection. In the present study, a yeast two-hybrid (Y2H) library was constructed to clarify the functions of wsv006, and the interaction between wsv006 and shrimp Litopenaeus vannamei (L. vannamei) was analyzed. Furthermore, we explored the role of the wsv006-interacting molecule L. vannamei COP9 constitutive photomorphogenic-like protein subunit 5 (LvCSN5) in WSSV infection. Y2H assay showed that wsv006 interacted with LvCSN5, and co-immunoprecipitation (Co-IP) assay confirmed such interaction. Multiple alignments of amino acid sequences with other species revealed that the LvCSN5 had high identity with Penaeusmonodon CSN5 (PmCSN5). LvCSN5 was mainly expressed in intestine, eye and hepatopancreas. In addition, the relative expression of LvCSN5 was significantly up-regulated both in intestine and hepatopancreas following the WSSV challenge. Besides, the relative expressions of IE1 and VP28, as well as the viral copy numbers were significantly increased in the LvCSN5-silenced shrimp. Our findings suggested that LvCSN5 was involved in WSSV infection by interacting with wsv006.

白斑综合症病毒（WSSV）作为一种毒性极强的病原体，极大地威胁着全世界的虾类水产养殖。病毒与宿主之间的相互作用对于病毒感染很重要。本研究通过构建酵母双杂交（Y2H）文库阐明wsv006的功能，并分析了wsv006与南美白对虾（L. vannamei）的相互作用。此外，我们探讨了 wsv006 相互作用分子南美白对虾COP9 组成型光形态发生样蛋白亚基 5 ( LvCSN5 ) 在 WSSV 感染中的作用。Y2H 分析表明 wsv006 与LvCSN5相互作用和免疫共沉淀 (Co-IP) 测定证实了这种相互作用。氨基酸序列与其他物种的多重比对表明，LvCSN5与单齿对虾CSN5（PmCSN5 ）具有高度同一性。LvCSN5主要在肠、眼和肝胰腺中表达。此外，在 WSSV 攻击后，LvCSN5在肠道和肝胰腺中的相对表达均显着上调。此外， IE1和VP28的相对表达量以及病毒拷贝数在LvCSN5沉默的虾中显着增加。我们的研究结果表明，LvCSN5通过与 wsv006 相互作用参与 WSSV 感染。