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AmAMP1 from Acropora millepora and damicornin define a family of coral-specific antimicrobial peptides related to the Shk toxins of sea anemones.
Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2020-09-13 , DOI: 10.1016/j.dci.2020.103866
B Mason 1 , I Cooke 2 , A Moya 1 , R Augustin 3 , M-F Lin 4 , N Satoh 5 , T C G Bosch 3 , D G Bourne 6 , D C Hayward 7 , N Andrade 8 , S Forêt 9 , H Ying 7 , E E Ball 10 , D J Miller 11
Affiliation  

A candidate antimicrobial peptide (AmAMP1) was identified by searching the whole genome sequence of Acropora millepora for short (<125AA) cysteine-rich predicted proteins with an N-terminal signal peptide but lacking clear homologs in the SwissProt database. It resembled but was not closely related to damicornin, the only other known AMP from a coral, and was shown to be active against both Gram-negative and Gram-positive bacteria. These proteins define a family of AMPs present in corals and their close relatives, the Corallimorpharia, and are synthesised as preproproteins in which the C-terminal mature peptide contains a conserved arrangement of six cysteine residues. Consistent with the idea of a common origin for AMPs and toxins, this Cys motif is shared between the coral AMPs and the Shk neurotoxins of sea anemones. AmAMP1 is expressed at late stages of coral development, in ectodermal cells that resemble the “ganglion neurons” of Hydra, in which it has recently been demonstrated that a distinct AMP known as NDA-1 is expressed.



中文翻译:

来自 Acropora millepora 和 damicornin 的 AmAMP1 定义了与海葵的 Shk 毒素相关的珊瑚特异性抗菌肽家族。

通过搜索千叶卫城全基因组序列鉴定出候选抗菌肽(AmAMP1 简称(<125AA)富含半胱氨酸的预测蛋白质,具有 N 末端信号肽,但在 SwissProt 数据库中缺乏明确的同源物。它与达米考宁相似但没有密切关系,达米考宁是唯一一种来自珊瑚的已知 AMP,并且被证明对革兰氏阴性和革兰氏阳性细菌都有活性。这些蛋白质定义了存在于珊瑚及其近亲 Corallimorpharia 中的 AMP 家族,并被合成为前原蛋白,其中 C 末端成熟肽包含六个半胱氨酸残基的保守排列。与 AMP 和毒素的共同起源的想法一致,这种 Cys 基序在珊瑚 AMP 和海葵的 Shk 神经毒素之间共享。AmAMP1在珊瑚发育的晚期,在类似于Hydra的“神经节神经元”的外胚层细胞中表达,其中最近证明了一种称为 NDA-1 的独特 AMP 被表达。

更新日期:2020-09-21
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