Dysphania ambrosioides L. is a medicinal plant with anti-helmintic potential. The aim of this study was to evaluate separately the effect of light spectra and elicitors on Dysphania ambrosioides growth and volatile constituents in vitro. Thus, plantlets were first cultured under blue (B), red (R), white, combinations of B:R (1:1, 2:1, 1:2) from LEDs and fluorescent lamps. Secondly, nodal segments were inoculated in the medium supplemented with chitosan (0, 50, 100, 150, and 200 mg L^− 1) and salicylic acid (0, 3, 6, 9, and 12 mg L^− 1). After 40 days of cultivation, the growth parameters and chemical composition of volatile constituents were evaluated. The light spectra significantly influenced in vitro growth of D. ambrosioides. The best growth occured using white LED or a blue:red combination of 2:1. It was also observed that the blue LEDs inhibited the synthesis of Z-ascaridole, while fluorescent light promoted a greater conversion of α-terpinene into ascaridole. The elicitors, chitosan and salicylic acid had a negative effect on the growth of nodal segments. However, the highest Z-ascaridole content was obtained at 50 to 100 mg L^− 1 of chitosan and with 6 to 9 mg L^− 1 of salicylic acid. The present study demonstrates that shoots regenerated from nodal segments exposed to different light spectra or on MS medium containing chitosan and salicylic acid can exhibit an altered growth and increased volatile constituents of interest.

Dysphania ambrosioides L.是具有抗蠕虫潜力的药用植物。这项研究的目的是分别评估光谱和激发子在体外对非洲夜蛾的生长和挥发性成分的影响。因此，首先在来自LED和荧光灯的B：R（1：1，2：1，1：2）的蓝色（B），红色（R），白色组合下培养幼苗。其次，将节段接种在补充有壳聚糖（0、50、100、150和200 mg L ^-1）和水杨酸（0、3、6、9和12 mg L ^-1）的培养基中。培养40天后，评估挥发性成分的生长参数和化学组成。光谱显着影响了D. ambrosioides的体外生长。使用白色LED或2：1的蓝：红组合可以实现最佳的生长。还观察到蓝色LED抑制了Z- a啶酮的合成，而荧光促进了α-萜品烯向a啶酮的更大转化。引发剂，壳聚糖和水杨酸对节段的生长具有负面影响。但是，在50至100 mg L ^-1的壳聚糖和6至9 mg L ^-1的水杨酸中，Z- Z啶含量最高。本研究表明，从暴露于不同光谱或包含壳聚糖和水杨酸的MS介质上的节段再生的芽可以表现出生长的变化和感兴趣的挥发性成分的增加。