In this study, a novel strategy for manipulating fluorescence emission of organic dyes is reported, which is based on coupling optical properties of colloidal crystals (CCs) with responsive microgels. Firstly, SiO2 microspheres doped with three kinds of organic dyes (Rhodamine B, RhB; fluorescein isothiocyanate, FITC; commercial cationic Fluorescent Yellow, X-10GFF) were prepared and they were used as the cores to synthesize SiO2/PNIPAm complex microgels doped with organic dyes by free radical polymerization. Then 3D self-assembled CCs of the complex microgels were fabricated by centrifugation. The microstructure of the complex microgel was characterized by Fourier transform infrared (FTIR), transmission electron microscopy (TEM), thermogravimetric (TG), dynamic light scattering (DLS), and differential scanning calorimeter (DSC). Moreover, the effect of doping organic dyes and temperature on self-assembly of the microgels were investigated. Manipulation of fluorescence emission for organic dyes by CCs was studied by photoluminescence (PL) and UV-visible spectrophotometer (UV-Vis). The results indicate that, due to the soft and thermosensitive property of PNIPAm shell, continuous adjustment to the photonic bandgap (PBG) of the CCs can be achieved conveniently by varying concentrations of the complex microgels or ambient temperatures, and when PBG of the CCs overlaps with the fluorescence emission peak of the organic dye, fluorescence emission of the organic dye can be manipulated.

中文翻译：

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SiO2/PNIPAm复合微凝胶的制备、自组装及其对有机染料荧光发射的调控

在这项研究中，报道了一种操纵有机染料荧光发射的新策略，该策略基于胶体晶体 (CC) 与响应性微凝胶的耦合光学特性。首先，制备了掺杂三种有机染料（罗丹明B、RhB；异硫氰酸荧光素，FITC；商业阳离子荧光黄，X-10GFF）的SiO2微球，并以它们为核心合成了掺杂有机染料的SiO2/PNIPAm复合微凝胶。自由基聚合染料。然后通过离心制造复杂微凝胶的 3D 自组装 CC。复合微凝胶的微观结构通过傅里叶变换红外 (FTIR)、透射电子显微镜 (TEM)、热重 (TG)、动态光散射 (DLS) 和差示扫描量热仪 (DSC) 进行表征。而且，研究了掺杂有机染料和温度对微凝胶自组装的影响。通过光致发光 (PL) 和紫外-可见分光光度计 (UV-Vis) 研究了 CC 对有机染料荧光发射的操纵。结果表明，由于 PNIPAm 壳的柔软和热敏特性，当 CCs 的 PBG 重叠时，可以通过改变复合微凝胶的浓度或环境温度来方便地实现对 CCs 光子带隙 (PBG) 的连续调节利用有机染料的荧光发射峰，可以操纵有机染料的荧光发射。通过光致发光 (PL) 和紫外-可见分光光度计 (UV-Vis) 研究了 CC 对有机染料荧光发射的操纵。结果表明，由于 PNIPAm 壳的柔软和热敏特性，当 CCs 的 PBG 重叠时，可以通过改变复合微凝胶的浓度或环境温度来方便地实现对 CCs 光子带隙 (PBG) 的连续调节利用有机染料的荧光发射峰，可以操纵有机染料的荧光发射。通过光致发光 (PL) 和紫外-可见分光光度计 (UV-Vis) 研究了 CC 对有机染料荧光发射的操纵。结果表明，由于 PNIPAm 壳的柔软和热敏特性，当 CCs 的 PBG 重叠时，可以通过改变复合微凝胶的浓度或环境温度来方便地实现对 CCs 光子带隙 (PBG) 的连续调节利用有机染料的荧光发射峰，可以操纵有机染料的荧光发射。