RNA editing is common in terrestrial plants, especially in mitochondria and chloroplast. In the photosynthesis process, NAD dehydrogenase plays a very important role. Subunit 2 of NADH-dehydrogenase is one of the major subunits in NAD dehydrogenase complex. Using desert plant Calotropis (Calotropis procera), this study focuses on the RNA editing activity of ndhB based on light time. NdhB (NADH-dehydrogenase subunit 2) gene accession no. MK144329 was isolated from Calotropis procera genomic data (PRJNA292713). Additionally, using RNA-seq data, the cDNA of the ndhB gene of C. procera was isolated at three daylight periods, i.e., dawn (accession no. MK165161), at midday (accession no. MK165160), and pre-dusk (accession no. MK165159). Seven RNA editing sites have been found in several different positions (nucleotide no. C467, C586, C611, C737, C746, C830, and C1481) within the ndhB coding region. The rate of these alterations was deferentially edited across the three daylight periods. RNA editing rate of ndhB gene was highest at dawn, (87.5, 79.6, 78.5, 76, 68.6, 39.3, and 96.9%, respectively), less in midday (74.8, 54.1, 62.6, 47.4, 45.5, 47.4, and 93.4%, respectively), and less at pre-dusk (67, 52.6, 56.9, 40.1, 40.7, 33.2, and 90%, respectively), also all these sites were validated by qRT-PCR. The differential editing of chloroplast ndhB gene across light periods may be led to a somehow relations between the RNA editing and control of photosynthesis.

中文翻译：

---

光/热对Calotropis（Calotropis procera）的叶绿体NADH-质体醌氧化还原酶亚基2（ndhB）基因中RNA编辑的影响。

RNA编辑在陆生植物中很常见，尤其是在线粒体和叶绿体中。在光合作用过程中，NAD脱氢酶起着非常重要的作用。NADH脱氢酶的亚基2是NAD脱氢酶复合物中的主要亚基之一。这项研究使用荒漠植物Calotropis（Calotropis procera），着眼于基于光照时间的ndhB的RNA编辑活性。NdhB（NADH-脱氢酶亚基2）基因登录号。从proco procera基因组数据（PRJNA292713）中分离出MK144329。另外，利用RNA-seq数据，在三个白天，即黎明（登录号MK165161），中午（登录号MK165160）和黄昏（登录号），分离了proc。procera ndhB基因的cDNA。 （编号：MK165159）。在几个不同的位置发现了七个RNA编辑位点（核苷酸编号C467，C586，C611，C737，C746，C830和C1481）在ndhB编码区域内。在三个白天对这些变化的速率进行了差异编辑。ndhB基因的RNA编辑率在黎明时最高（分别为87.5％，79.6％，78.5％，76％，68.6％，39.3％和96.9％），中午时较少（74.8％，54.1％，62.6％，47.4％，45.5％，47.4％和93.4％ ，以及在黄昏前较少（分别为67％，52.6％，56.9％，40.1％，40.7％，33.2％和90％），所有这些位点均通过qRT-PCR进行了验证。跨光周期叶绿体ndhB基因的差异编辑可能导致RNA编辑与光合作用控制之间的某种关系。分别在午间减少（分别为74.8％，54.1、62.6、47.4、45.5、47.4和93.4％），黄昏前较少（分别为67、52.6、56.9、40.1、40.7、33.2和90％） ），所有这些位点均通过qRT-PCR进行了验证。跨光周期叶绿体ndhB基因的差异编辑可能导致RNA编辑与光合作用控制之间的某种关系。分别在午间减少（分别为74.8％，54.1、62.6、47.4、45.5、47.4和93.4％），黄昏前较少（分别为67、52.6、56.9、40.1、40.7、33.2和90％） ），所有这些位点均通过qRT-PCR进行了验证。跨光周期叶绿体ndhB基因的差异编辑可能导致RNA编辑与光合作用控制之间的某种关系。