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An in vitro screening potential traditional medicinal plants for nephrolithiasis
Clinical Phytoscience Pub Date : 2020-09-12 , DOI: 10.1186/s40816-020-00209-5
Majid Shirani , Davood Arjaki , Soleiman Kheiri , Elham Bijad , Sareh Mohammadi , Zahra Lorigooini

Today, the attention of researchers has been drawn to the use of medicinal plant for the treatment of kidney stones. The aim of this study was to investigate the effect of five plants used in traditional medicine on the dissolution of calcium oxalate and calcium phosphate stones. Then, the ability of more effective plants to dissolve stones collected from patients after Percutaneous Nephrolithotripsy was investigated. The aerial part of plants were extracted by maceration method. The synthesized stones in laboratory were incubated with different concentrations of the extract. Next, the concentrations of calcium oxalate and calcium phosphate were measured by a calcium kit and BT 3000. The effect of the extract with the best activity on the stones collected from the patients was also studied. The composition of clinical stones was determined by colorimetric method. The total phenolic content (TPC) of the extracts was determined. The highest dissolution of calcium oxalate stones was observed by the G. struthium extract and the highest dissolution of calcium phosphate stones by the A. euchroma and A. officinalis root extracts. The dissolution percentage of clinical stones by the A. euchroma extract was significantly higher than other extract (P < 0.05). The highest TPC was observed in A. euchroma extract (P < 0.05). The A. euchroma extract exhibited the greatest dissolution activity on laboratory calcium oxalate and calcium phosphate stones as well as clinical stones made of high amounts of calcium oxalate. Therefore, the extract can be effective in preventing and treating kidney stones.

中文翻译:

体外筛选潜在的传统药用植物的肾结石病

如今,研究人员已将注意力转向使用药用植物治疗肾结石。这项研究的目的是研究传统医学中使用的五种植物对草酸钙和磷酸钙结石溶解的影响。然后,研究了更有效的植物溶解经皮肾镜碎石术后从患者身上收集的结石的能力。用浸软法提取植物的地上部分。将实验室合成的宝石与不同浓度的提取物一起孵育。接下来,用钙试剂盒和BT 3000测量草酸钙和磷酸钙的浓度。还研究了活性最高的提取物对从患者身上收集的结石的影响。用比色法测定临床结石的成分。测定提取物的总酚含量(TPC)。草uth提取物观察到草酸钙结石的最高溶解,常绿草和厚朴根提取物观察到磷酸钙结石的最高溶解。常绿曲霉提取物对临床结石的溶解百分比显着高于其他提取物(P <0.05)。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。测定提取物的总酚含量(TPC)。草uth提取物观察到草酸钙结石的最高溶解,常绿草和厚朴根提取物观察到磷酸钙结石的最高溶解。常绿曲霉提取物对临床结石的溶解百分比显着高于其他提取物(P <0.05)。在常青曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。测定提取物的总酚含量(TPC)。草uth提取物观察到草酸钙结石的最高溶解,常绿草和厚朴根提取物观察到磷酸钙结石的最高溶解。常绿曲霉提取物对临床结石的溶解百分比显着高于其他提取物(P <0.05)。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。chrom提取物和常青草和厚朴根提取物对磷酸钙结石的溶解度最高。常绿曲霉提取物对临床结石的溶解百分比显着高于其他提取物(P <0.05)。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。uth提取物和常青草和厚朴根提取物对磷酸钙结石的溶解度最高。常绿曲霉提取物对临床结石的溶解百分比显着高于其他提取物(P <0.05)。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。在常绿曲霉提取物中观察到最高的TPC(P <0.05)。常绿曲霉提取物对实验室草酸钙和磷酸钙结石以及由大量草酸钙制成的临床结石表现出最大的溶解活性。因此,该提取物可以有效地预防和治疗肾结石。
更新日期:2020-09-12
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