Background:Drought stress affects grapevine growth and development and reduces berry yield and quality. Identifying genes that are involved in the plant response to drought stress will enable the development of new grape strains that are tolerant to drought. Objective:We cloned the VvibZIP14 gene from Vitis vinifera and analyzed its role in drought resistance. Methods:Gene expression was analyzed by quantitative real-time PCR. Subcellular localization was assessed with a transient expression assay. The transactivation activity of the protein was evaluated in yeast. The physiologic role of VvibZIP14 was analyzed by overexpressing VvibZIP14 in Arabidopsis following drought stress. Hydrogen peroxide accumulation in Arabidopsis was visualized by diaminobenzidine staining. Results:Drought stress caused the accumulation of VvibZIP14, which was localized in the nucleus and had transcriptional activity. Transgenic plants showed improved resistance to drought stress and reduced electrolyte leakage compared to plants overexpressing empty vector, whereas chlorophyll content, photosystem II maximal photochemical efficiency, and net photosynthetic rate were higher. Catalase, peroxidase, and superoxide dismutase activities were also increased in VvibZIP14-overexpressing plants subjected to drought stress. Conclusions:VvibZIP14 functions as a transcription factor that confers resistance to drought stress in grape by enhancing the antioxidant response.

中文翻译：

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葡萄（Vitis vinifera）bZIP14充当转录激活因子，并通过抑制活性氧来增强抗旱性

背景：干旱胁迫影响葡萄的生长发育并降低浆果产量和品质。鉴定涉及植物对干旱胁迫的响应的基因将使得能够开发耐受干旱的新葡萄菌株。目的：从葡萄中克隆VvibZIP14基因，分析其在抗旱中的作用。方法：通过实时定量PCR分析基因表达。用瞬时表达测定法评估亚细胞定位。在酵母中评估了蛋白质的反式激活活性。通过在干旱胁迫后在拟南芥中过表达VvibZIP14来分析VvibZIP14的生理作用。通过二氨基联苯胺染色观察过氧化氢在拟南芥中的积累。结果：干旱胁迫导致VvibZIP14积累，它位于细胞核中并具有转录活性。与过表达空载体的植物相比，转基因植物显示出对干旱胁迫的改善的抵抗力和减少的电解质渗漏，而叶绿素含量，光系统II的最大光化学效率和净光合速​​率更高。在遭受干旱胁迫的过表达VvibZIP14的植物中，过氧化氢酶，过氧化物酶和超氧化物歧化酶的活性也增加了。结论：VvibZIP14可以作为转录因子，通过增强抗氧化作用，赋予葡萄抗旱性。光系统Ⅱ的最大光化学效率和净光合速​​率均较高。在遭受干旱胁迫的过表达VvibZIP14的植物中，过氧化氢酶，过氧化物酶和超氧化物歧化酶的活性也增加了。结论：VvibZIP14是转录因子，可通过增强抗氧化反应而赋予葡萄抗旱性。光系统Ⅱ的最大光化学效率和净光合速​​率均较高。在遭受干旱胁迫的过表达VvibZIP14的植物中，过氧化氢酶，过氧化物酶和超氧化物歧化酶的活性也增加了。结论：VvibZIP14是转录因子，可通过增强抗氧化反应而赋予葡萄抗旱性。