Abstract

In the current scenario nanoparticles (NPs) have gained a breathtaking impetus due to their multidimensional applications in varied fields. In the present effort, biogenic synthesis of Zinc Oxide nanoparticles (ZnO NPs) was carried out using aqueous extract of dried powder of Emblica officinalis (Amla). Physicochemical characterization of nanoparticles was carried out via UV-Visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) wherein the particles were found to be quasi spherical and with a size ranging between 3 and 11 nm. The ZnO nanoparticles exhibited significant antibacterial activity against bacteria as Streptococcus pyogenes MTCC 442, Bacillus cereus MTCC 1272, Escherichia coli MTCC 1687 and Pseudomonas aeruginosa MTCC 4673. The nanoparticles displayed high anti-biofilm activity toward all the bacterial strains, when tested against three different base materials viz. glass, plastic and metal (Aluminum). Further, the nanoparticle treatment of bacterial cells caused changes in their cell membrane permeability, leading to leakage of nucleic acid from the bacterial cells, thereby defining it as the most probable mechanism for their anti-biofilm potential.

摘要

在当前情况下，由于纳米颗粒（NPs）在不同领域中的多维应用，获得了惊人的动力。在目前的努力中，氧化锌纳米颗粒（ZnO NPs）的生物合成是使用Emblica officinalis（Amla）干燥粉末的水性提取物进行的。纳米颗粒的理化特性通过紫外可见光谱，傅里叶变换红外光谱，红外光谱，扫描电子显微镜和透射电子显微镜进行表征。被发现是准球形的，大小在3至11 nm之间。ZnO纳米颗粒对化脓性链球菌MTCC 442具有显着的抗菌作用，蜡状芽孢杆菌MTCC 1272，大肠杆菌MTCC 1687和铜绿假单胞菌MTCC4673。当针对三种不同的基础材料进行测试时，纳米粒子对所有细菌菌株均显示出高抗生物膜活性。玻璃，塑料和金属（铝）。此外，细菌细胞的纳米颗粒处理引起其细胞膜通透性的变化，从而导致核酸从细菌细胞中泄漏，从而将其定义为其抗生物膜潜力的最可能机制。