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Effect of Sublethal Blue Light on Herbal Extract Activity Against Volatile Sulfide Compound Production by Fusobacterium nucleatum
Photochemistry and Photobiology ( IF 3.3 ) Pub Date : 2020-09-25 , DOI: 10.1111/php.13332
Uziel Jeffet 1 , Neta Dagan 1 , Nir Sterer 1
Affiliation  

Previously, we have shown that sub-lethal exposure of blue light caused increased cell membrane permeability in Fusobacterium nucleatum. The aim of the present study was to test the effect of this exposure on the activity of Lavender, Sage, Echinacea and Mastic gum extracts against volatile sulfide compounds (VSC) production by Fusobacterium nucleatum. Bacterial suspensions were pre-exposed to blue light (400-500nm) bellow minimal inhibitory dosage (sub-MID). Exposed and non-exposed samples were inoculated into test tubes containing growth medium, filtered saliva with or without herbal extracts. Following incubation test tubes were tested for malodor production (Odor judge scores), VSC levels (OralChroma), salivary protein degradation (SDS-PAGE) and bacterial cells membrane damage (fluorescence microscopy). Results showed that sub-MID blue light exposure significantly increased the ability of Lavender and Echinacea to reduce VSC production by Fusobacterium nucleatum by more than 30%. These results suggest that sub-lethal blue light exposure may be useful to increase the efficacy of anti-malodor agents.

中文翻译:

亚致死蓝光对有核梭杆菌生产挥发性硫化物化合物的草本提取物活性的影响

以前,我们已经表明,亚致死的蓝光暴露会导致具核梭杆菌的细胞膜通透性增加。本研究的目的是测试这种暴露对薰衣草、鼠尾草、紫锥菊和乳香提取物对具核梭杆菌产生的挥发性硫化物 (VSC) 活性的影响。将细菌悬浮液预先暴露于低于最小抑制剂量 (sub-MID) 的蓝光 (400-500nm)。将暴露和未暴露的样品接种到含有生长培养基、过滤唾液的试管中,其中含有或不含草药提取物。孵育后测试试管的恶臭产生(气味判断评分)、VSC 水平(OralChroma)、唾液蛋白降解(SDS-PAGE)和细菌细胞膜损伤(荧光显微镜)。结果表明,亚中度蓝光照射显着增加了薰衣草和紫锥菊减少具核梭杆菌产生的 VSC 的能力超过 30%。这些结果表明,亚致死蓝光暴露可能有助于提高抗恶臭剂的功效。
更新日期:2020-09-25
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